[1]吴建忠.利用正交设计优化亚麻SRAP-PCR反应体系[J].黑龙江农业科学,2012,(03):30-32.
　WU Jian-zhong.Optimization of SRAP-PCR System for Flax Based on Orthogonal Design[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):30-32.
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利用正交设计优化亚麻SRAP-PCR反应体系

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2012年03 页码: 30-32 栏目: 出版日期: 2012-03-01
Title:
Optimization of SRAP-PCR System for Flax Based on Orthogonal Design
文章编号:
1002-2767（2012）03-0030-03
作者:
吴建忠
黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086
Author(s):
WU Jian-zhong
Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086
关键词:
亚麻; 正交设计; 优化; SRAP
Keywords:
flax; orthogonal design; optimization; SRAP
分类号:
S563.2
文献标志码:
A
摘要:
为亚麻分子标记及分子育种提供可靠的理论依据，利用正交试验设计对亚麻SRAP-PCR反应体系中的4因素（模板DNA、引物浓度、Mg2+浓度、dNTPs浓度）在3个水平上进行正交优化，确立了适合亚麻SRAP-PCR反应的20 μL体系：75 ng模板DNA、0.75 μmol·L-1引物、1.5 mmol·L-1 Mg2+、0.40 mmol·L-1dNTPs和1.5 U Taq DNA聚合酶。
Abstract:
In order to provide reliable theory basis for flax molecular marker and molecular breeding，the orthogonal design was used to optimize SRAP-PCR amplification system at 3 levels of 4 factors in flax（DNA template，primer，Mg2+，dNTPs）.The most suitable SRAP-PCR reaction system for flax was total 20 μL containing 75 ng DNA template，0.75 μmol·L-1 primers，1.5 mmol·L-1 Mg2+，0.40 mmol·L-1 dNTPs and Taq DNA polymerase 1.5 U.

参考文献/References:

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[6]王斌，党占海，张建平，等.亚麻SRAP反应体系的优化[J].基因组学与应用生物学，2009，28(4):760-764.

[7]吴建忠.亚麻全基因组DNA的提取及分析[J].黑龙江农业科学，2011(7):18-19.

[8]刘立军，蒙祖庆，邢秀龙，等．苎麻基因组SRAP扩增体系的优化研究[J]．分子植物育种，2006，4（5）：726-730.


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备注/Memo

作者简介：吴建忠（1983-），男，内蒙古自治区乌兰察布市人，硕士，研究实习员，从事亚麻生物技术及遗传育种研究。E-mail：wujianzhong176@163.com。

更新日期/Last Update: 2014-10-26