REN Jia-yun,SHAN Chang-qing,XU Lan-juan,et al.Effects of PCB1254 on Osmotic Adjustmentof the Scallop Chlamys ferrar[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(12):79-82.
多氯联苯(PCB1254)对栉孔扇贝渗透调节的影响
- Title:
- Effects of PCB1254 on Osmotic Adjustmentof the Scallop Chlamys ferrar
- 文章编号:
- 1002-2767（2011）12-0079-04
- 关键词:
- 多氯联苯; 栉孔扇贝; 渗透压; Na+-K+-ATPase
- Keywords:
- PCB1254; Chlamys ferrari; osmotic pressure; Na+-K+-ATPase
- 分类号:
- X171
- 文献标志码:
- A
- 摘要:
- 为了给我国沿海有机物污染地区栉孔扇贝健康养殖提供监测依据，研究了多氯联苯（PCB1254）对栉孔扇贝血淋巴渗透压和鳃丝Na+-K+-ATPase活力的影响。结果表明：PCB1254浓度为1.0 μg·L-1以上处理组栉孔扇贝血淋巴渗透压变化明显，其中1.0 μg·L-1处理组扇贝血淋巴渗透压显著上升，而10.0和50.0 μg·L-1处理组栉孔扇贝的血淋巴渗透压呈先上升后下降的趋势，最后与海水对照组相近；低浓度（0.5和1.0 μg?L-1）处理组扇贝鳃丝Na+-K+-ATPase活力明显被激活，而高浓度（10.0和50.0 μg·L-1）处理组Na+-K+-ATPase活力在测定的每个时间点明显被抑制。因此认为多氯联苯影响下，机体所产生的有害代谢物可导致栉孔扇贝应激性反应和细胞膜结构的改变，导致血淋巴渗透压和Na+-K+-ATPase活力的变化，而且Na+-K+-ATPase活力变化与PCB1254浓度呈现一定相关性，能反映机体受PCB1254影响的程度，可以作为评价PCB1254污染的生理学指标。
- Abstract:
- In order to provide monitoring criterion for scallops healthy breeding in organic matter pollution area of China’s coastal,the effects of PCB1254（0.5，1.0，10.0 and 50.0 μg·L-1） on osmotic adjustment of the scallop Chlamys ferrari were studied.The results showed that the osmotic pressure of hemolymph changed significantly under the PCB1254 of all concentrations except for the 0.5 μg·L-1treatment.The osmotic pressure increased significantly during all experimental time in 1.0 μg·L-1,while the osmotic pressure increased soon and then decreased nearly to the seawater control levels in 10.0 μg·L-1 and 50.0 μg·L-1 groups.The Na+-K+-ATPase activity of gills of Chlamys ferrari increased significantly in low concentrations(0.5 μg·L-1 and 1.0 μg·L-1)of PCB1254,but it was restrained by high concentrations of PCB1254 during all sampling time.It concluded that the metabolic production produced by PCB1254 could cause the excitation response and the changes of cell membrane of the scallop Chlamys ferrari,so the osmotic pressure and Na+-K+-ATPase activity changed.The changes of Na+-K+-ATPase activity showed certain regularity,it could reflect the whole ability of detoxification of the organism as well as the damage degrees caused by PCB1254,so it could be used as the evaluated biomarker for toxicity of PCB1254.
参考文献/References:
[1]储少刚,徐晓白,童逸平.多氯联苯在典型污染地区环境中的分布及其环境行为[J].环境科学学报,1995,15(4):42-43. [2]聂湘平,张凤君,蓝崇玉.多氯联苯对斑马鱼(Brachydanio rerio)毒性及其组织结构的影响[J].生态科学,2004,23(2):106-109. [3]Richard H M E，Wouter Z,Cor A S,et al.Effects of PCB126 and Cadmium on the Anaerobic Metabolism of the Mussel Mytilus edulis?L.[J].Com.Biochem.Physiol,1996,113(2):267-272. [4]Whealty M G,Hentry R P.Branchial and antennal Na+/K+- dependent ATPase and carbonie anhydrase activity during salinity acclimation of the euryhaline crayfish Pacifastacus leniuscndus[J].J.Exp.Biol.,1987,133:73-86. [5]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal.Biochem.,1976,72(1):248-254. [6]吕富.环境因子对中华绒螯蟹渗透调节的影响[D].青岛：中国海洋大学,2002. [7]卢敬让,赖伟.镉对中华绒螯蟹鳃组织及其亚显微结构的影响[J].海洋与湖沼,1991,22（6）:566-571. [8]Bjerregaard,Vislie P T.Effects of mercury on ion and osmoregulation in the shore crab Carcinus maenas(L.)[J].Comparative Biochemistry and Physiology,1985,82:227-230. [9]Johnson I T,Jones M B.Effect of zinc on osmoregulation of Gammarus duebeni(Crustacea:amphipoda)from the estuary and the sewage treatment works at Looe[J].Cornwall.-Ophelia,1990,31:187-196. [10]沈同，王镜岩.生物化学[M].北京：高等教育出版社，1990. [11]Wang Zhaohui,Yin Yiwei,Zhang Yongyuan.Effect of fenpropathrin on ATPase activity and card ia muscle con traction of silver carp(Hypoph thalm ichthy smoli trix)[C]//Annual Report of State Key Laboratory for Freshwater Ecology and Biotechnology of China.Beijing:International Academic Publisher,1992. [12]Yap H H，Desaiah K,Cutkomp L K,et al.in votro?inhibition of fish brain ATPase activity by cyclodiene insecticides and related compounds[J].Bull Environ Contam Toxicol,1975,14(2):163-167. [13]Boese B L,Johnson V G,Chapman D E,et al.Effects of petroleum refinery waste water expsure on gill ATPase and selected blood parameters in the pacific staghorn sculpin(Leptocottus armatus)[J].Comp.Biochem Physiol.,1982,71:63-67. [14]谭晓珍,吴垠,李韬,等.氯氰菊酯和氰戊菊酯对栉孔扇贝的急性毒性[J].大连水产学院学报,2005,2(3):203-207. [15]徐镜波，景体凇.硝基芳烃对鲤鱼鱼鳃ATPase的活性抑制和QSARs[J].中国环境科学,1998,18（2）:158-161. [16]袁锦芳，王忠，陈淑龙，等.TBTCI对鲤鱼组织Na+-K+-ATPase的毒性研究[J].南开大学学报：自然科学，2001,34(2):104-108. [17]黄周英,陈奕欣,赵扬,等.三丁基锡对文蛤鳃酸性磷酸酶、碱性磷酸酶和Na+-K+-ATPase酶活性的影响[J].海洋环境科学,2005,24(3):56-59.
备注/Memo
基金项目： 滨州学院青年人才创新工程资助项目（BZXYQNLG200505）