[1]黄九林,魏春雁,李庆华.连翘不同部位连翘苷含量测定及其抗氧化活性的测定[J].黑龙江农业科学,2011,(11):84-86.
　HUANG Jiu-lin,WEI Chun-yan,LI Qing-hua.Study on Content of Phillyrin and the Antioxidative Capacity of Different Parts of Forsythia suspensa [J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):84-86.
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连翘不同部位连翘苷含量测定及其抗氧化活性的测定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2011年11 页码: 84-86 栏目: 出版日期: 2011-11-10
Title:
Study on Content of Phillyrin and the Antioxidative Capacity of Different Parts of Forsythia suspensa
文章编号:
1002-2767201111-0084-03
作者:
黄九林1; 魏春雁2; 李庆华3
1.安康学院，陕西 安康725000；
2.吉林省农业科学院 农业环境与资源中心，吉林 长春 130033；
3.吉林医药学院 医学实验中心，吉林 吉林 132013
Author(s):
HUANG Jiu-lin1; WEI Chun-yan2; LI Qing-hua3
1.Ankang College，Ankang，Shananxi，725000;
2.Agricultural Environment and Resource Center of Jilin Academy of Agricultural Sciences，Changchun，Jilin，130033
3.Jilin Medical College，Jilin，Jilin，132013
关键词:
连翘; 超声波辅助法; 连翘苷; 抗氧化
Keywords:
Forsythia suspensa; ultrasonic extraction; phillyrin; antioxidation
分类号:
S685.24
文献标志码:
A
摘要:
为综合利用连翘，现采用超声波辅助法提取连翘苷，HPLC法测定连翘苷，DPPH自由基清除法评价抗氧化活性，对测定连翘不同部位提取液连翘苷含量及其抗氧化活性进行了研究。结果表明：超声波辅助提取法的最佳提取条件为甲醇含量75%，提取30 min，物料比为8∶1，提取次数为2次；连翘叶中连翘苷含量显著高于连翘花和果实；连翘花、果实、叶提取液抗氧化活性较强。表明连翘苷在连翘叶中含量较高，连翘不同部位提取液都具有较强的抗氧化活性。
Abstract:
Inorder to determinethe content of phillyrin and the antioxidant activities of different parts of Forsythia suspensa，ultrasonic extraction was applied to extrat phillyrin,the antioxidant activities were assayed through scavenging effects to DPPH radical，and the content of forsythin of Forsythia suspensa?was determined by HPLC.The results showed that the best extraction condition was the methyl alcohol content 75%，withdraws 30 minutes，the material ratio was 8∶1，the extraction number of times was twice.The content of phillyrin in forsythia leaf was higher than forsythia flower and fruit obviously.Both different parts of Forsythia suspensa had antioxidant activity.So the content of phillyrin in forsythia leaf was higher and different parts of Forsythia suspensa?had antioxidant activity.

参考文献/References:

[1]中国科学院北京植物所.高等植物图鉴:第三册[M].北京：科学出版社，1974.

[2]曲欢欢，李白雪，燕菲,等.用清除有机自由基DPPH法评价连翘不同部位抗氧化作用[J].中国中药信息杂志，2008(S1):32-34.

[3]Larrauri J A，Sanchez M C，Saura C F.Effect of temperature on the free radical scavenging capacity of extracts from red and white grape pomace peels[J].Agric Food Chem,1998，46：2694.

[4]Paniwnyk L,Beaufoy E,Lorimer J P,et al.The extraction of rutin from flower buds of Sophora japonica[J].Ultrason Sonochem,2001,8:299-301.


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备注/Memo

第一作者简介：黄九林(1981-)，男，四川省广安市人，硕士，讲师，从事天然产物研究。E-mail：huangjiulin@yahoo.com.cn。

更新日期/Last Update: 2014-11-01