WANG Nan-nan.Analysis of Soybean on Oil Content with QTL Marker[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(04):1-3.
大豆油分含量QTL分析
- Title:
- Analysis of Soybean on Oil Content with QTL Marker
- 文章编号:
- 1002-2767（2011）04-0001-03
- Keywords:
- oil content of soybean; quality trait; QTL; SSR
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以美国半矮秆大豆品种Charleston与高蛋白品系东农594杂交得到的154株RILs群体作为试验材料，利用在亲本之间表现多态的SSR引物对群体进行分析，根据已有的遗传图谱，利用浙江大学朱军的QTLmaper2.0对各年各点的数据进行分析。结果表明：根据油分含量LOD值（≥2.50）的大小，确定6个QTL位点位于3条连锁群上。Qsoil 1、Qsoil 2、Qsoil 3分别位于遗传图谱GM1-A1连锁群Satt449-Qsoil 1 、Qsoil 2-Satt545，Satt571-Qsoil 3-Satt522四个引物之间。Qsoil 4、Qsoil 5位于遗传图谱GM3-B1连锁群Satt229-Qsoil 4-Satt197和Sat_113-Qsoil 5-sat_099四个引物之间。Qsoil 6位于遗传图谱GM2-A2连锁群Satt327和Satt468两个引物之间。
- Abstract:
- Taking 154 RILs which were obtained by crossbreeding between semi-dwarf cultivar Charleston from America and the high protein soybean stain Dongnong 594 as materials，using SSR primers which manifest polymorphism in parents to analyze the population，according to the genetic map，and using the QTLmaper2.0 which was invented by Zhu Jun of Zhejiang University to analyze the date of points and years，the six QTLs of content of oil were confirmed located three linkage groups based on the value of LOD.The result showed that Qsoil 1，Qsoil 2、Qsoil 3 located GM1-A1 linkage group and among the four primers Satt449-Qsoil 1，Qsoil 2-Satt545，Satt571-Qsoil 3-Satt522.Qsoil 4，Qsoil 5 located GM3-B1 linkage group and among the four primers Satt229-Qsoil 4-Satt197 and Sat_113-Qsoil 5-sat_099.Qsoil 6 located GM2-A2 linkage group and among the two primers Satt327 and Satt468.
参考文献/References:
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备注/Memo
基金项目：国家“863”计划资助项目(2006AA10Z1F4)