PENG Hong-yu,YU Li-jie.Integration Pattern and Character of Exogenous Genesin Transgenic Plants[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(01):25-27.
植物转基因的整合特性及遗传分析
- Title:
- Integration Pattern and Character of Exogenous Genesin Transgenic Plants
- 文章编号:
- 1002-2767201101-0025-03
- Keywords:
- transgenic plants; exogenous genes; integration; inheritance
- 分类号:
- Q943.2
- 文献标志码:
- A
- 摘要:
- 对转基因植物的外源基因整合到受体染色体上的整合机制,整合随机性与非随进性,整合位点及拷贝数等特性进行了综述。并对3种外源基因遗传规律的方式进行了分析,它们分别是:单基因位点single-site的孟德尔遗传方式、多基因位点multi-site的孟德尔遗传方式和非孟德尔non-Mendel的遗传方式。
- Abstract:
- The integration of exogenous genes in transgenic plants was described,and the characters of its mechanism,randomness and non-randomness,integration site and copy number of exogenous genes integration were summerized.Then,three ways in which rule of inheritance could be achieved:single-site Mendel’s law of inheritance,multi-site Mendel’s law of inheritance,and non-Mendel’s law of inheritance.
参考文献/References:
[1]Hernandez P , Barcelo P , Lazzeri P A , et al.Morphological and agronomic variation in transgenic tritordeum lines grown in the field[J].J.Plant Phsiol , 2000 , 156:223 - 229. [2] Rasco-Gaunt S,Riley A,Cannell M,et al.Procedures allowing the transformation of a range of European elite wheatTriticum aestivum?L.varieties via particle bombardment[J].J.Exp Bot,2001,357(52):865-874. [3] Kohli A,Leech M,Vain F,et al.Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots[J].Proc Natl Acad Sci USA,1998,95(12):7203-7208. [4] Svitashev S K,Somers D A.Characterization of transgene loci in plants using FISH:A picture is worth a thousand words[J].Plant Cell,Tissue and Organ Culture,2002,69(3):205-214. [5] Makarevitch I,Svitashev S K,Somers D A.Complete sequence analysis of transgene loci from plants transformed via microprojectile bombardment[J].Plant Mol Biol,2003,52(2):421-432. [6] 赵艳,于彦春,黄大年.植物中转基因的整合机制[J].中国生物工程杂质,2003,23(2):29-32. [7] Choi H W,Lemaux P G,Cho M J.Use of fluorescence in situ hybridization for gross mapping of transgenes and screening for homozygous plants in transgenic barleyHordeum vulgare?L.[J].Theor Appl Genet,2002,106(1):92-100. [8] Altpter F,Baisakh N,Beachy R,et al.Particle bombardment and the genetic enhancement of crops:myths and realities[J].Mol Breeding,2005,15(3):305-327. [9] 徐明良,杨金水,葛扣麟.植物转基因的整合机制[J].植物生理学通讯,1996,32(3):234-240. [10] 曾凡锁,詹亚光.转基因植物中外源基因的整合特性及其研究策略[J].植物学通报,2004,21(5):565-577. [11] Forsbach A,Schubert D,Lechtenberg B,et al.A comprehensive characterization of single-copy T-DNA insertions in the Arabidopsis thaliana genome[J].Plant Mol Biol,2003,52(1):161-176. [12] Svitashev S K,Somers D A.Genomic interspersions determine the size and complexity of transgene loci in transgenic plants produced by microprojectile bombardment[J].Genome,2001,44(4):691-697. [13] Vaira A M,Berio T,Accotto G P,et al.Evaluation of resistance in Osteospermum ecklonisDC.Norl.Plants transgenic for the N protein gene of tomato spotted wilt virus[J].Plant Cell Rep,2000,19:983-988. [14] Svitashev S,Ananiev E,Pawlowski W P,et al.Association of transgene integration sites with chromosome rearrangements in hexaploid oat[J].Theor Appl Genet,2000,100(6):872-880. [15] 陶利珍,凌定厚,张世平.基因枪介导的籼稻遗传转化及外源基因在受体中的遗传研究[J].武汉植物学研究,1999,17(4):289-296. [16] 姚方印,朱常香,李广贤,等.Bt水稻的抗虫性鉴定及转基因的遗传分析[J].中国农业科学,2002,35(2):144-145. [17] Zhang S,Warkentin D,Sun B,et al.Variation in the inheritance of expression among subclones for unselectedUidAand SelectedBartransgenes in maizeZea maysL.[J].Theoretical and Applied Genetics,1996,92:752-761. [18] Yin Z,Plader W,Malepszy S.Transgene inheritance in plants[J].J.Appl.Genet.,2004,45:127-144. [19] 周静,弭晓菊,崔继哲.植物转基因的非孟德尔遗传[J].分子植物育种,2006,4(5):614-620.
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备注/Memo
第一作者简介:彭洪雨(1985-),女,黑龙江省拜泉县人,在读硕士,从事转基因植物的分子生物学研究。E-mail:penghy624@126.com。