CAI Quan,CAO Jing-sheng,SHI Gui-rong,et al.Rapid Testing Bt Protein from Maize by Bt Test Paper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):133-134.
利用Bt试纸快速检测玉米中的Bt蛋白
- Title:
- Rapid Testing Bt Protein from Maize by Bt Test Paper
- 文章编号:
- 1002-2767(2010)10-0137-02
- Keywords:
- maize(Zea mays L.); Bt test paper; transgenic Bt; Bt protein
- 分类号:
- S513
- 文献标志码:
- A
- 摘要:
- 利用少量玉米幼苗嫩叶,加双蒸水和石英砂快速研磨,制成混合液,离心后取上清液插入Bt试纸条,可以在20 min之内检测出玉米中是否含有Bt蛋白,该方法快速、准确、简单易行,可以大量检测玉米样品,显著提高转Bt基因玉米的检测效率,对于商检、海关和农业执法等部门具有重要的实用价值,同时也对从事农作物转Bt基因研究的科研人员检测Bt蛋白提供帮助。
- Abstract:
- Using some young leaf from seeding of maize,adding ddH2O and quartz sand,then grinding the mixture fast,after centrifugate the mixture,insert the Bt test paper into the supernatant,the result whether contain Bt protain would appear in 20 minutes.The method is fast,accurate and easy to operate,so its very practical to commercial test,customs and law-enforcement of agriculture.Meanwhile,it could help scientific researchers teste the Bt protein in the transgenic Bt crops.
参考文献/References:
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[2]李桂玲,李明泽,刘宗华,等.转Bt基因抗虫玉米的研究[J].生物技术通报,2009(5):9-13.
[3]张书红,席章营.快速提取玉米叶片DNA的新方法[J].安徽农业科学,2007,35(13):3376,3801.
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备注/Memo
基金项目:转基因生物新品种培育重大专项资助项目(2008zx08003-001)