GUO Yi-fan,ZHANG Hong-ji,SUN Yan,et al.Variance Analysis of Microsatellite Markers in Spring Wheat SP2 Induced by Space Flight[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(07):9-12,16.
春小麦空间诱变SP2的SSR标记变异分析
- Title:
- Variance Analysis of Microsatellite Markers in Spring Wheat SP2 Induced by Space Flight
- 文章编号:
- 1002-2767（201007-0009-04
- 分类号:
- S512
- 文献标志码:
- A
- 摘要:
- 为了考察空间搭载对小麦的诱变效果，以实践八号育种卫星搭载的小麦品系为试材，对SP2的DNA分子标记突变频率进行分析。结果表明：12对SSR引物共扩增出3种突变类型：扩增片段增多、扩增片段减少和扩增片段长度有差异。出现的单一突变类型居多，在同一品系的个体上同时出现多类型突变的情况很少。突变频率在供试的品系之间以及SSR染色体位点都存在差异。该研究又以7个EST-SSR标记对上述SP2个体的扩增结果显示，与一般的SSR位点相比，EST-SSR标记检测到的突变类型单一，仅有“扩增片段增多”一种类型，且突变频率普遍较低，大部分基因位点无突变发生。表明空间搭载对小麦基因组产生的诱变主要发生在重复序列区，基因表达区相对较少。
- Abstract:
- In order to study wheat induced by space flight，using the wheat by seed-breeding satellite，Shijian-8 us experimental materials，the wheat SP2 DNA molecular marker mutation frequency were determined.The results showed that 12 groups SSR primers amplified all 3 types of mutations：increased fragments amplification，decreased amplified fragment and amplified fragment length difference.A single mutation majority，in the same type of individual strains on the situation drastically mutation appears at the same time.Mutation frequency in the test strains and SSR chromosome loci was different.While this study through 7 EST-SSR on the individuals expansion SP2，the results showed that compared with the general SSR sites，EST-SSR marker loci of detecting the mutation was simple：a single type of mutation，only ‘amplification increased a type’fragments，and mutations in the low frequency statistics，most without mutation genetic loci.Space flight launch on wheat of mutation occurs mainly in the repeat sequences，but gene expression area was relatively rare.
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备注/Memo
“十一五”国家科技支撑计划资助项目(2008BAD97B01)；黑龙江省科技计划资助项目(GA09B101-4-3)；黑龙江省博士后启动金资助项目(LBH2Q06008)