JIANG Wei-dong.Optimization and Primer Screening of the ISSR Reaction System in Flax[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(05):10-12.
亚麻ISSR的反应体系优化及引物筛选
- Title:
- Optimization and Primer Screening of the ISSR Reaction System in Flax
- 文章编号:
- S563.2
- Keywords:
- flax; genome DNA; ISSR reaction; screening
- 分类号:
- S563.2
- 文献标志码:
- A
- 摘要:
- 对亚麻ISSR反应条件进行了优化。同时利用3个有代表性的亚麻样品从60条ISSR引物中筛选出13条多态性好、重复性高的引物，为亚麻的分子鉴定奠定了基础。
- Abstract:
- The ISSR reaction conditions of flax were optimized. 13 primers out of 60 were screened out by 3 typical flax varieties which had high level polymorphism and repeatability. This research made the foundation for the molecular identification of flax.
参考文献/References:
[1]Zietkiewiecz E, Rafalske A, Iabuda D. Genome fingerprinting by simple sequence repeat(SSR) anchored polymerase, chain reaction amplyficatlon[J]. Genome, 1994, 20:178 - 183. [2]Fritsch P, Riese L H. The use of random amplified poly-morphic DNA(RAPD) in conservation geneties[M]//Smith T B，Wayne R K. Molecular Genetic Approaches in Conservation. London:Oxford University Press, 1996:54-73. [3]Hedrick P W. Conservation genetics and molecular techniques:A pespective[M]//Smith T B，Wayne R K. Molecular Genetic Approaches in Conservation. London:Oxford University Press,1996:459-477. [4]CHEN Xiang-ming, ZHENG Guo-sheng, MENG Li. RAPD-PCR analysis of genetic diversity of different colour 35 tree paeonia cultivars[J].Sci Agric Sin, 2002,35(5):546-551. [5]LI Jin-bo,JIANG Liang-rong,LI Chun-hai, et al. Comparisions of genetic analysis based on ISSR and SSR in PGMS and TGMS rice lines[J]. Moecul Plant Breed,2003,1 (1):42-47. [6]萨姆布鲁克J,拉塞尔D W.分子克隆实验指南[M]. 黄培堂，金冬雁，黎孟枫，译.北京：科学出版社,2002.
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备注/Memo
基金项目：〖JP2〗哈尔滨市科技攻关计划资助项目（2008AA6CH06）