JIA Xiang-nan,XIN Bei,MA Sheng-jun,et al.Comparison Study on the Content of Endogenous Hormones in Transgenic Double Genes of Populus tomentosa Carr. during the Rooting Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(05):7-9，,12.
转双价基因毛白杨组培生根过程内源激素含量对比研究
- Title:
- Comparison Study on the Content of Endogenous Hormones in Transgenic Double Genes of Populus tomentosa Carr. during the Rooting Culture
- 文章编号:
- 1002-2767（2010）05-0007-03
- 分类号:
- S567.23+1
- 文献标志码:
- A
- 摘要:
- 为探讨转rolB-pttGA20ox双价基因毛白杨生根生理变化，对转rolB-pttGA20ox双价基因毛白杨株系RG-4和对照株系PT-16组培苗生根过程中内源激素含量进行了测定分析。结果表明：RG-4株系在无激素培养基上8 d即可见根突出现，比对照提前3 d，生根率、平均根长及根数均高于对照；其内源IAA含量在不定根形成时增加，根形成后降低，且RG-4在起始和处于峰值时期的内源IAA含量显著高于对照；RG-4的GA3含量在生根前降低，生根后升高，与对照的变化趋势相反；ABA含量在生根过程中波动变化，但起始ABA含量RG-4低于PT-16；RG-4的IAA/ABA值在根突形成前保持较高的水平，根突形成后降低。
- Abstract:
- AbstractIn order to study the rooting and physiological changes of the transgenic rolB-pttGA20ox double genes Populus tomentosa Carr., the content of endogenous hormones in the controlled strains PT-16 and the transgenic rolB - pttGA20ox?double genes Populus tomentosa Carr. RG-4 were measured. The results showed that in the hormone-free medium, root processus of RG-4 formed in 8 days, 3 days earlier than the controlled strains, and the rooting rate, the average length and the number of roots were also higher than those of the controlled strains. The content of endogenous IAA in RG-4 was rising during the formation of adventitious root, but decreasing after the process, and the content in the beginning and peak time were significantly higher than that of the controlled strains. The content of GA3 in RG-4 was decreasing before the root formation while increasing after the root formation, which was just opposite to the controlled strains. The content of ABA fluctuated during the root formation, but at the beginning, the content of ABA in RG-4 was lower than that in PT-16. The ratio of IAA and ABA of RG-4 kept a high level before the formation of root processus, but decreased after the process.
参考文献/References:
[1]Maldiney R, Pelese F, Pilate G, et al. Endogenous levels of abscisic acid, indole-3-acetic acid, zeatin and zeatin-riboside during the course of adventitious root formation in cuttings of Craigella and Craigella lateral suppressor tomatoes[J]. Pysiol Plant, 1986, 68: 426-430. [2]徐继忠,陈四维.桃硬枝插条的内源激素（ABA，IAA）含量变化对生根的影响[J].园艺学报，1989,16（4）:275-278. [3]刘关君，李绪尧，由香玲，等．长白落叶松插穗内源激素变化与不定根产生的关系[J]．东北林业大学学报，2000，28(1)：19-20. [4]肖关丽，杨清辉，李富生．甘蔗组培苗继代培养中内源激素与绿苗生根率关系研究[J]．云南农业大学学报，2001,16(4)：271-273. [5]郑钧宝,裴保华,耿桂荣.毛白杨扦插生根的研究[J].东北林业大学学报, 1988, 16(6): 34-40. [6]赵一宇.河北杨插条内源激素的季节性消长与生根的关系[J].林业科学,1987,23(2):208-213. [7]蒙海涛,韩清芳,贾志宽,等.水培紫花苜蓿的茎段生根过程中内源激素含量变化[J]．植物生理学通讯,2008,44(3):498-500. [8]陈雪梅,高红兵,王沙生.三种杨树扦插生根期间内源激素水平的比较研究[J].林业科学,1994,30(1):1-7. [9]辛蓓.rolB-pttGA20ox 双价基因转化毛白杨及遗传稳定性研究[D].北京:北京林业大学,2009. [10]于雪飞, 白卉, 王洪星.4种内源激素在中美山杨组培生根过程中的变化[J].林业科技,2009,34（2）:5-7. [11]郑钧宝,刘玉军,裴保华,等.木本植物茎插穗生根于内源激素的关系[M]//王沙生，王世绩，裴保华.杨树栽培生理研究. 北京：北京农业大学出版社,1991:360-367. [12]Estruch J J, Schell J, Spena A. The protein enconded by the rolB plant oncogene hydrolyses indole glucosides[J].Embo J ., 1991, 10(1):3125-3128. [13]Spena A, Estruch J J, Prinsen E, et al. Anther-specific expression of the rolB gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth[J]. Theor Appl Genet , 1992, 84:520-527.
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备注/Memo
基金项目：国家自然科学基金资助项目(30271097)