[1]王旭达,丰 明,李景鹏.优化水稻培养条件测定其果胶酶活力及灰度变化[J].黑龙江农业科学,2010,(03):5-7.
　WANG Xu-da,FENG Ming,LI Jing-peng.Optimize the Condition of Rice Cultivation and Determinate the Change of the Pectinase Activity and the Gray-scale[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(03):5-7.
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优化水稻培养条件测定其果胶酶活力及灰度变化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2010年03 页码: 5-7 栏目: 出版日期: 2010-03-10
Title:
Optimize the Condition of Rice Cultivation and Determinate the Change of the Pectinase Activity and the Gray-scale
文章编号:
1002-2767（2010）03-0005-03
作者:
王旭达; 丰 明; 李景鹏
辽宁省农业科学院 大连生物技术研究所，辽宁 大连 116024
Author(s):
WANG Xu-da; FENG Ming; LI Jing-peng
Dalian Biotechnology Institute of Liaoning Academy of Agricultural Sciences,Dalian,Liaoning 116024
关键词:
发芽水稻; 变性淀粉; 果胶酶活力; 灰度
Keywords:
germination rice; modified starch; activity of pectinase;  gray-scale
分类号:
S511
文献标志码:
A
摘要:
采用浸泡发芽培养方法改变水稻分子内部构象，测定其变性淀粉果胶酶活力的变化，选择最佳浸泡发芽条件并测定变性后淀粉灰度。结果表明：最佳培养条件为浸泡温度25℃，浸泡方式为浸泡4 h断水10 h，加碱量为0.03%Ca(OH)2，培养时间为2.5 d，在25℃条件下发芽6 d，变性淀粉果胶酶活性最高，灰度较好。从而成功实现对发芽水稻变性淀粉果胶酶活性及其灰度分布的测定。
Abstract:
By soaking the rice for the cultivation the molecular conformation changed,and determinated the change of the pectinase activity,then,we determinated the best immersion and germination conditions and the gray-scale.Results:The best conditions for cultivate was that immersion temperature was 25℃,immersion mode was 4 hours immersion and 10 hours un-immersion,immersion alkali content was 0.03% Ca(OH)2, immersion time was 2.5 days, germination temperature was 25℃,germination time was 6 days, the pectinase activity and the gray-scale of the modified starch were best at this time.Conclusion: the pectinase activity and the gray-scale of rice modified starch were determinated successfully.

参考文献/References:

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[2]Meakin P.Fractal, Sealing and Growth far from E-quilibrium[M]. Cambridge:Cambridge Universit Press,2000.

[3]洪雁,顾正彪.变性淀粉新工艺新技术及新产品的发展方向[J].淀粉与淀粉糖,2006(2):17.

[4]尤新.淀粉衍生物[M].北京:中国物资出版社,2001：176.

[5]李兆丰,顾正彪.酸解氧化淀粉的制备及其性质研究[J].食品与发酵工业,2005,31(11):411-415.

备注/Memo

第一作者简介：王旭达（1982-），男，黑龙江省绥化市人，硕士，研究实习员，从事生物技术和微生物学研究。E-mail：wangxuda860@sina.com。

更新日期/Last Update: 2014-11-04