[1]张君朝,任传英,黄文功.米糠和豆粕中植酸的提取及含量的测定[J].黑龙江农业科学,2009,(02):132-134.
　ZHANGJun-zhao,REN Chuan-ying,HUANG Wen-gong.Extraction and Determination of Phytic Acid in the RiceBran and Bean Pulp[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(02):132-134.
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米糠和豆粕中植酸的提取及含量的测定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年02 页码: 132-134 栏目: 出版日期: 2009-03-10
Title:
Extraction and Determination of Phytic Acid in the RiceBran and Bean Pulp
文章编号:
1002-2767(2009)02-0132-03
作者:
张君朝1 ;  任传英2 ;  黄文功3
(1 .哈高科大豆食品有限责任公司, 黑龙江哈尔滨150078;2 .黑龙江省哈高科营养食品有限公司, 黑龙江哈尔滨150078 ;3.黑龙江省农业科学院经济作物研究所, 哈尔滨150086)
Author(s):
ZHANGJun-zhao1 ;  REN Chuan-ying2 ;  HUANG Wen-gong3
1 .Hi-Tech Soybean Food Co ., Ltd , Harbin , Heilongjiang 150078 ;2 .Heilongjiang Hi-Tech Nut rimentFood Co ., Ltd,Harbin ,Heilongjiang 150078 ;3 .Indust rial Crops Institute of Heilongjiang Academy of AgriculturalSciences ,Harbin ,Heilongjiang 150086
关键词:
豆粕; 米糠; 植酸含量; 阴离子交换树脂;  测铁分光光度法
Keywords:
bean pulp; rice bran; the content of phytic acid; anion exchange resin; measure iron pectrophotometric method
分类号:
S816.4
文献标志码:
A
摘要:
为了提取米糠和豆粕中的植酸并测定其含量, 采用阴离子交换树脂提取植酸, 测铁分光光度法测定植酸含量。结果表明, 影响提取率的主要因素有提取时间、提取温度、树脂类型及分离时的流速。通过正交试验确定了最佳的提取、分离工艺:提取温度45℃、提取时间3 h、D201 大孔阴离子交换树脂、流速1 mL·min-1 , 测得米糠中提取植酸含量为70.39 mg·g-1 , 豆粕中提取植酸含量为21.14 mg·g-1 。该提取方法提取率高, 测定方法操作简单、准确度较高, 适用于农副产品中植酸的提取及含量的定量测定。
Abstract:
Anion exchange resin was used to extract phytic acid and measure iron pectrophotometric method was adopted todetermine phytic acid concentration.The results showed that:Extraction time, extractio n temperature, resin type and flowrate were main influencing factors.The optimal craft were that ex traction temperature was 45 ℃, ex traction time was 3 h,D201 anion exchange resin was adapted to disassociation, flow rate was 1 mL per min.Phy tic acid content were 70.39mg·g-1 and 21.14 mg·g-1 by the method in the rice and bean pulp respectively .It could be concluded that theext raction method w as ef fective and the assay method was simple ,accurate , they w ere adapt to extractionand determine the phy tic acid in the agriculture by-product .

参考文献/References:

[ 1] 　Green E S ,Zangerl A R, Berenbaum M R.Ef fect s of Phytic Acid andXanthot oxin on Grow th and Det oxification in Cat erpillars[ J] .Journalof Chemical Ecology , 2001 , 27(9):1763-1773.

[ 2] 　William D Hitz , Thomas J Carlson ,Phil S Kerr , et al.Biochemical andMolecular Characterization of a MutationThat Conf ers a DecreasedRaf finosaccharide and Phytic Acid Phenot ype on Soybean Seeds[ J] .Plant Physiology, 2002, 128 :650-660.
[ 3] 　Cynthia B Andaya , Thomas H Tai .Fine mapping of the ri ce low phyticacid(Lpa1)locus[ J] .TAG Theoret ical and Applied Genetics ,2005 ,111(3):489-495.
[ 4] 　朱文适, 袁庆云, 胡蓉.米糠生产植酸和肌醇的工艺条件[ J] .山地农业生物学报,1999 ,18(4):235-238 .
[ 5] 　李丹, 崔洪斌.植酸及其生物学活性研究现状[ J] .国外医学卫生学分册, 2004, 31(2):104-108 .
[ 6] 　刘坤, 高华, 张东峰, 等.不同植酸测定方法的对比分析[ J] .青岛医学院学报, 1997, 33(3):191-192 .
[ 7] 　GB/ T5009.153 -2003 ,植物性食品中植酸的测定[ S] .

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备注/Memo

第一作者简介:张君朝(1968- ),女, 辽宁辽阳人, 学士, 中级, 从事食品分析研究。Tel :13936552620;E-mail:zjz5393@163 .com。

更新日期/Last Update: 2016-08-23