ZHANGFeng-sheng,FAN Shao-zhu,XUE Dan-dan,et al.Study on Leaves of Kim Jong-il Begonia tuberhybrieda VosaInduced by Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(02):17-19.
金正日球根海棠叶片组织培养诱导的研究
- Title:
- Study on Leaves of Kim Jong-il Begonia tuberhybrieda VosaInduced by Tissue Culture
- 文章编号:
- 1002-2767(2009)02-0017-03
- Keywords:
- Begonia tuberhybrieda Vosa ; tissue culture ; induction
- 分类号:
- S685.99
- 文献标志码:
- A
- 摘要:
- 以金正日球根海棠(Begonia tuberhybrieda Vosa)的叶片为外植体进行组织培养的诱导, 以Ms 培养基为基本培养基, 并附加不同浓度的细胞分裂素6-BA 以及生长素NAA 或2 , 4-D 进行组织培养和植株再生。经实验筛选出最适合诱导愈伤组织的培养基是MS +6-BA1.0 ～ 2.0 +NAA0.1 ～ 0.5 ;脱分化的最佳激素组合是培养基MS +6-BA1.0 +NAA0.5。
- Abstract:
- Leaves of Kim Jong-il Begonia tuberhybrieda Vo sa were used as explants to conduct tissue culture induction.Msmedium was basic medium, adding with different concentrations of cytokinin 6-BA and NAA or 2 , 4-D to carry on plant tissueculture and regeneration.The experiment selected the most suitable callus induction of the medium was MS +BA1.0～2.0+NAA0.1～ 0.5;dedifferentiation hormone combination was the best medium MS+BA1.0+NAA0.5.
参考文献/References:
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备注/Memo
收稿日期:2008-08-04第一作者简介:张凤生(1977-), 男, 黑龙江绥化市人, 学士, 农艺师,从事园艺类组织培养研究。Tel:13936322142 ;0451-84302560 。