DENG Ke.Effect of Different Ratio of Hormones on TissueCulture of Dendrobium candidum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(06):19-20.[doi:10.11942/j.issn1002-2767.2008.06.0019]
不同激素配比对铁皮石斛组培的影响
- Title:
- Effect of Different Ratio of Hormones on TissueCulture of Dendrobium candidum
- 文章编号:
- 1002-2767(2008)06-0019-02
- Keywords:
- Dendrobium candi dum ; tissue culture ; callus
- 分类号:
- S567
- 文献标志码:
- A
- 摘要:
- 通过多次实验研究, 力图找出不同基本培养基和不同浓度激素对铁皮石斛组织快繁时愈伤组织诱导的影响。研究结果表明:诱导愈伤组织时, 在MS 、B5 、N6 、RM 这4 种基本培养基中, 以N6 基本培养基对愈伤组织的诱导效果最好;添加4 种不同浓度的6-BA, 浓度为1 .0 mg·L -1 时诱导愈伤组织效果最好。
- Abstract:
- The study w as striving to find out the effect of inducing of callus fo rmatio n during the rapid pro pag atio nin Dendrobi um candidum , with differe nt ba sical cultur e medium and concentra tion o f ho rmones .The resultsshowed that the o ptimal inducing medium w as made of N6 among the diffe rent inducing medium of M S , B5 , N6 ,RM w hen inducing of callus fo rmation , mo reov er , the best effo rt w as adding 1 mg · L-1 of 6-BA among the fourvaries co ncentra tion.
参考文献/References:
[ 1] 徐建华, 陈立钻, 李莉,等.铁皮石斛与西洋参的养阴生津作用研究[ J] .中草药, 1995 , 26(2):79-80 .
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备注/Memo
基金项目:南阳师范学院项目(nyct200405)