[1]苏　萍,戴常军,任红波,等.电泳条件的优化与玉米贮藏蛋白聚丙稀酰胺凝胶电泳[J].黑龙江农业科学,2002,(02):21-22.[doi:10.11942/j.issn1002-2767.2002.02.0021]
　SU Ping,DAI Chang-jun,REN Hong-bo,et al.Combination of Electrophoretic Factors and Polyacrylamide Gel Electrophoresis for Maize Storage Proteins[J].HEILONGJIANG AGRICULTURAL SCIENCES,2002,(02):21-22.[doi:10.11942/j.issn1002-2767.2002.02.0021]
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电泳条件的优化与玉米贮藏蛋白聚丙稀酰胺凝胶电泳

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2002年02 页码: 21-22 栏目: 出版日期: 2002-03-10
Title:
Combination of Electrophoretic Factors and Polyacrylamide Gel Electrophoresis for Maize Storage Proteins
文章编号:
1002 -2767(2002)02 -0021 -02
作者:
苏　萍;  戴常军;  任红波;  高春霞
黑龙江省农科院谷物品质研究中心, 哈尔滨150086
Author(s):
SU Ping ;  DAI Chang-jun;  REN Hong-bo ;  GAO Chun-xia
Center of Cereal Quality , Heilongjiang Academy of Agricultural Sciences , Harbin 150086 , China
关键词:
玉米; 贮藏蛋白; 聚丙烯酰胺凝胶电泳
Keywords:
maize ; storage proteins ; polyacrylanmide gel electrophoresis
分类号:
S513 .01
DOI:
10.11942/j.issn1002-2767.2002.02.0021
文献标志码:
A
摘要:
研究了影响玉米贮藏蛋白不连续聚丙烯酰胺凝胶电泳分离效果的主要因素。结果表明, 分离胶缓冲液的离子强度、pH 值、电极缓冲液的离子强度和胶浓度是影响分离效果的主要因素, 催化剂用量对分离效果影响不大。
Abstract:
An acidic discontinuous polyacrylamide gel electrophoretic system for maize storage protein separationwas established in our laboratory .Factors affecting the resultant resolution were investigated .The resultsindicated that ionic intensity of the buffer and pH of the gels , the concentration of the separating gel had significanteffect on protein bands resolution , the amount of the catalysts was the least of all .

参考文献/References:

[ 1] Wang Chun , Bi an Ke , Zhang Hua Xiao , et al.Polyacrylamide gel electrophoresisof salt -soluble proteins for maize variety identifi cation andgenetic purity assessment [ J] .Seed Science and Technology , 1994 , 22 :51-57 .

[ 2] Wang Hong-Xin , Niu Zhan-Qi , Hu Zhi-Ang.Electrophoreti c study onseed proteins of maize hybrids and inbreds[ J] .Chinese J Bot , 1989 , 1 :139-144 .
[ 3] 张建华, 刘正蒙, 孟凡义.电泳技术在品种鉴别和种子纯度鉴定中的应用[ J] .中国农学通报1992 ,8(5):14-15.
[ 4] 周展明, 卞科, 王春, 等.玉米蛋白PAGE 法鉴定玉米品种及纯度[ J].郑州粮食学院学报,1992 ,(3):23-29.[ 5] 　宋同明, 郑大浩, 刘岩, 等.利用玉米种子白蛋白和球蛋白乳酸聚丙烯酰胺电泳鉴定品种[ J] .植物学报, 1996 , 38(8):599-604.

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备注/Memo

收稿日期:2001 -09-06　

基金项目:黑龙江省自然科学基金(C9744)资助项目　
作者简介:苏萍(1966 -), 女, 河北乐亭人, 副研究员, 硕士, 从事谷物品种研究。

更新日期/Last Update: 2016-12-04