[1]华晓琴,马鸿文,俞天泉,等.石羊河流域中下游柠条锦鸡儿种群遗传结构分析[J].黑龙江农业科学,2025,(04):61-67.[doi:10.11942/j.issn1002-2767.2025.04.0061]
　HUA Xiaoqin,MA Hongwen,YU Tianquan,et al.Genetic Structure Analysis of Caragana korshinskii Population in Middle and Lower Reaches of Shiyang River Basin[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(04):61-67.[doi:10.11942/j.issn1002-2767.2025.04.0061]
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石羊河流域中下游柠条锦鸡儿种群遗传结构分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2025年04 页码: 61-67 栏目: 出版日期: 2025-04-10
Title:
Genetic Structure Analysis of Caragana korshinskii Population in Middle and Lower Reaches of Shiyang River Basin
文章编号:
10
作者:
华晓琴1; 2; 马鸿文1; 2; 俞天泉1; 2; 闫海旺3; 李浩4
（1.武威市国家杨树樟子松良种基地, 甘肃 武威 733000； 2.武威市林业综合服务中心, 甘肃 武威 733000； 3.古浪县林业技术服务中心, 甘肃 武威 733100； 4.北京林业大学 林学院,北京 100083）
Author(s):
HUA Xiaoqin1; 2;  MA Hongwen1; 2;  YU Tianquan1; 2;  YAN Haiwang3; LI Hao4
(1.Wuwei National Poplar and Camphor Pine Breeding Base, Wuwei 733000,China; 2.Wuwei Forestry Comprehensive Service Center, Wuwei 733000,China; 3.Gulang County Forestry Technology Service Center, Wuwei 733100,China; 4.College of Forestry,Beijng Forestry University, Beijing 100083,China）
关键词:
石羊河流域中下游; 柠条锦鸡儿; 单倍型; ITS序列; 群体遗传多样性
Keywords:
middle and lower reaches of the Shiyang River Basin;  Caragana korshinskii;  haplotype;  ITS sequence;  population genetic diversity
DOI:
10.11942/j.issn1002-2767.2025.04.0061
文献标志码:
A
摘要:
为研究石羊河流域中下游5个柠条锦鸡儿(Caragana korshinskii)种群的遗传结构和多样性，利用ITS与 psbA-trnH 联合序列对石羊河流域中下游5个柠条锦鸡儿种群中样本进行物种鉴定，再对5个柠条锦鸡儿种群的遗传结构和遗传多样性进行分析。结果表明，（1）5个种群中优势种为柠条锦鸡儿；（2）柠条锦鸡儿种群共存在20个单倍型，以原始单倍型Hap1为节点形成了一个大分支，以Hap9、Hap10和Hap12为节点形成了3个小分支，其中，阿拉古山种群中单倍型最为丰富，阿拉古山种群与扎子沟种群中单倍型突变方向差异最大；（3）柠条锦鸡儿群体间变异为14.28%，群体内的变异为85.72%，柠条锦鸡儿群体分化指数在0.02~0.27之间。群体间存在遗传分化，其中，八步沙种群与阿拉古山、扎子沟种群间的群体遗传分化最大。由此可见，柠条锦鸡儿阿拉古山种群、扎子沟种群、八步沙种群群体遗传结构存在差异，可以作为下一步柠条锦鸡儿育种的种质材料。
Abstract:
In order to reveal the population genetic structure and diversity of five Caragana korshinskii populations in the middle and lower reaches of the Shiyang River Basin, ITS and psbA-trnH sequences were used to identify samples and analyzed population genetic structure and population genetic diversity from five populations of C. korshinskii in the middle and lower reaches of the Shiyang River Basin. The results showed that, (1) The dominant species in the five populations was C. korshinskii. (2) A total of 20 haplotypes existed in the C. korshinskii population, with the original haplotype Hap1 as the node to form a large branch, and Hap9, Hap10, and Hap12 as the nodes to form three sub-branches, among which the Alagu Mountain population haplotypes was the most abundant, and the difference in the direction of the haplotype mutation was the greatest between the Alagu Mountain population and the Zhazigou population. (3) The inter-population variation of C. korshinskii was 14.28% and intra-population variation accounted for 85.72%. The Fst of C. korshinskii population ranged from 0.02 to 0.27, and genetic differentiation existed among populations, with the greatest population genetic differentiation between the Babusha population and the Alagu Mountain and Zhazigou populations. In conclusion, it can be seen that, there are differences in the genetic structure of the populations of C. korshinskiifrom Alagu Mountain, Zhazigou, and Babusha, which can be used as germplasm materials for the next breeding of C. korshinskii.

备注/Memo

收稿日期：2025-02-25

基金项目：甘肃省青年基金计划（22JR5RH1034）；2022年甘肃省林业和草原科技项目（2022kj015）。
第一作者：华晓琴（1988-），女，硕士，高级工程师，从事林木良种选育研究。E-mail：278689104@qq.com。

更新日期/Last Update: 2025-04-10