ZHANG Jie,CHEN Yongqi,SHI Yihan,et al.Nodules Microbiome of Mung Bean in Cold Regions and Isolation, Re-Inoculate Activity of Dominant Bradyrhizobium japonicum from Mung Bean Nodules[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(02):7-14.[doi:10.11942/j.issn1002-2767.2025.02.0007]
寒区绿豆根瘤微生物组及优势慢生根瘤菌的分离、结瘤活性
- Title:
- Nodules Microbiome of Mung Bean in Cold Regions and Isolation, Re-Inoculate Activity of Dominant Bradyrhizobium japonicum from Mung Bean Nodules
- 文章编号:
- 2
- Keywords:
- mung bean; nodule; Microbiome; Bradyrhizobium japonicum; reinoculate test
- 文献标志码:
- A
- 摘要:
- 为探究寒区绿豆根瘤的微生物组并获得绿豆的本地根瘤菌资源，利用高通量测序方法分析了黑龙江省大庆市安达试验田绿豆根瘤的微生物组，并利用平板划线法从绿豆根瘤中分离、鉴定优势根瘤菌，并开展了回接结瘤试验，考察了结瘤植株的生长情况。结果显示，绿豆根瘤中共检测到113个OTU，主要微生物菌群来自变形菌门、蓝藻门、放线菌门和未分类细菌门，优势的慢生根瘤菌属占64.29%，其他为非根瘤菌属。网络分析显示慢生根瘤菌属与6种非根瘤菌存在相关性，并与假单胞菌属存在正相关性。分离获得的优势根瘤菌利用16S rDNA进行分子鉴定结果表明,该根瘤菌与慢生根瘤菌(Bradyrhizobium japonicum)的相似性为99.93%。回接结瘤后的绿豆植株根长减少5.8%，但其株高以及鲜重增加39.7%和236.1%，地下鲜重提高111.4%。
- Abstract:
- In order to investigate the nodules microbial community of mung bean in cold regions and obtain local rhizobium resources for mung beans, nodules microbiome of mung bean was analyzed using high-throughput sequencing methods, and a rhizobia was isolated and identified from nodules of mung bean in Anda experimental field, Daqing City, Heilongjiang Province by the plate streaking method. Moreover, we conducted a reinoculate test to investigate the growth of plants. The results showed that 113 OTUs were detected in the nodules of mung bean and the dominant phyla included Proteobacteria, Cyanobacteria, Actinobacteria, and Unclassified Bacteria; The dominant Bradyrhizobium genus accounted for 64.29%, while the others are non-rhizobia genera. The network analysis showed a correlation between Bradyrhizobium and six non-rhizobia, and a positive correlation between Bradyrhizobium and Pseudomonas. The molecular identification based on 16S rDNA showed the rhizobia had a 99.93% similarity with Bradyrhizobium japonicum. After nodulation, the root length of mung bean decreased by 5.8%, but the plant height and fresh weight increased by 39.7% and 236.1%, respectively. The underground fresh weight increased by 111.4%. This study analyzed the endophytic community structure of mung bean nodules and obtained a dominant Bradyrhizobium japonicum with growth promoting ability from the nodules.
相似文献/References:
[1]邢宝龙,冯 高,郭新文,等.绿豆旱区高产群体结构与功能优化初报[J].黑龙江农业科学,2012,(03):38.
XING Bao-long,FENG Gao,GUO Xin-wen,et al.Preliminary Report on High Yield Population Structure and Function Optimization of Mung Bean in Arid Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(02):38.
[2]李 敏,李清泉,曾玲玲.黑龙江省绿豆生产存在的问题及对策[J].黑龙江农业科学,2011,(10):133.
LI Min,LI Qing-quan,ZENG Ling-ling.Problems and Countermeasures in Production ofMung Bean in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):133.
[3]曾玲玲,刘 峰,崔秀辉,等.几种杀菌剂防治绿豆根腐病田间药效试验分析[J].黑龙江农业科学,2011,(09):45.
ZENG Ling-ling,LIU Feng,CUI Xiu-hui,et al.Analysis on the Field Tests of Several Kinds of Fungicides to Prevent Root Rot in Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):45.
[4]王 成,刘 峰.绿豆田除草剂筛选试验[J].黑龙江农业科学,2011,(09):54.
WANG Cheng,LIU Feng.Screening Test of Herbicides in Mung Bean Field[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(02):54.
[5]李建英,田中艳,周长军,等.绿豆芽菜萌发条件及物质含量测定[J].黑龙江农业科学,2010,(07):37.
LI Jian-ying,TIAN Zhong-yan,ZHOU Chang-jun,et al.Study on Germination and Determination of Material Content of Sprout Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(02):37.
[6]曾玲玲,崔秀辉,李清泉,等.氮磷钾配施对绿豆产量的效应研究[J].黑龙江农业科学,2010,(07):48.
ZENG Ling-ling,CUI Xiu-hui,LI Qing-quan,et al.Studies on the Effects of Application Ratios of Nitrogen，Phosphorus and Potassium on Yield of Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(02):48.
[7]马长山.21%氟磺.烯草酮油悬浮剂对绿豆田一年生杂草防效研究[J].黑龙江农业科学,2014,(08):59.
MA Chang-shan.Control Efficacy of 21% Fluorosulfonylimide.Clethodim oil SC on Weeds in Mung Bean Field[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):59.
[8]于运凯.玉米绿豆间作效应分析[J].黑龙江农业科学,2014,(02):29.
YU Yun kai.Effects of Intercropping for Maize and Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):29.
[9]贾学方,王超.绿豆耐盐愈伤组织的诱导[J].黑龙江农业科学,2015,(08):10.[doi:10.11942/j.issn1002-2767.2015.08.0010]
JIA Xue fang,WANG Chao.Induction of Salttolerant Callus in Mung Bean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(02):10.[doi:10.11942/j.issn1002-2767.2015.08.0010]
[10]宋国权,戴新文.辽西半干旱地区绿豆高产栽培技术[J].黑龙江农业科学,2009,(05):174.[doi:10.11942/j.isn1002-2767.2009.05.0174]
SONGGuo-quan,DAI Xin-wen.High Yield Culture Technique of Mung Bean in Half Arid Area ofWestern of Liaoning Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(02):174.[doi:10.11942/j.isn1002-2767.2009.05.0174]
备注/Memo
收稿日期：2024-10-14