[1]杨帆,王爽,蒋希峰,等.拮抗内生真菌非生物胁迫抗性及抑菌效果评价[J].黑龙江农业科学,2025,(02):1-6.[doi:10.11942/j.issn1002-2767.2025.02.0001]
　YANG Fan,WANG Shuang,JIANG Xifeng,et al.Evaluation of Abiotic Tolerance and Inhibition Effects of Antagonistic Endophytic Fungi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(02):1-6.[doi:10.11942/j.issn1002-2767.2025.02.0001]
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拮抗内生真菌非生物胁迫抗性及抑菌效果评价

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2025年02 页码: 1-6 栏目: 出版日期: 2025-02-10
Title:
Evaluation of Abiotic Tolerance and Inhibition Effects of Antagonistic Endophytic Fungi
文章编号:
1
作者:
杨帆1;  王爽1;  蒋希峰1;  杜虹锐1;  李沐恺2;  刘春来1
（1.黑龙江省农业科学院 植物保护研究所/农业农村部哈尔滨作物有害生物科学观测实验站,黑龙江 哈尔滨 150086； 2.黑龙江八一农垦大学 农学院，黑龙江 大庆 163319）
Author(s):
YANG Fan1;  WANG Shuang1;  JIANG Xifeng1;  DU Hongrui1;  LI Mukai2;  LIU Chunlai1
(1.Plant Protection Institute, Heilongjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Crop Pest in Harbin, Ministry of Agriculture and Rural Affairs, Harbin 150086, China； 2.College of Agriculture, Heilongjiang Bayi Agricultural Reclamation University, Daqing 163319, China)
关键词:
内生真菌; 非生物胁迫; 抗逆性; 抑菌作用
Keywords:
endophytic fungi;  abiotic stress;  stress resistance;  inhibition effect on phytopathogen
DOI:
10.11942/j.issn1002-2767.2025.02.0001
文献标志码:
A
摘要:
为挖掘利用优良拮抗菌资源，选取前期初筛试验中对不同靶标菌表现有拮抗作用的菌株，进行复筛试验。测定了9株拮抗内生真菌在盐渍化、温度差异化逆境胁迫下，菌株的生长情况。结果表明，青霉属菌株1-R-6对6种靶标菌均有不同程度的抑制作用，表现出广谱性。离蠕孢属菌株2-Fr-8及棘壳孢属菌株12-R-5分别对玉米大斑病菌及灰葡萄孢菌有较强的抑制作用，抑菌率分别为50.79%和66.67%，具有潜在的生防潜力。蓝状菌属菌株11-R-10表现出较强的抗高温特性，40 ℃条件下，平皿测定菌落直径可达3.72 cm；4株内生真菌在4 ℃条件下缓慢生长，且镰刀菌属菌株16-FL-1的菌落直径也可达到1.06 cm。菌株16-FL-1在含盐量3%的平皿中菌丝生长与对照组无差异，且在12%的含盐平皿条件下菌落直径仍可达到2.66 cm，表现出较强的抗盐渍化能力。
Abstract:
In order to explore and utilize excellent antagonistic isolates resources, the strains that exhibited antagonistic effects against different target pathogens in the preliminary screening experiment were selected and a re-screening test was conducted. Nine antagonistic endophytic fungi were tested for their growth under stress conditions of salinization and different temperatures. The results showed that Penicillium strain 1-R-6 exhibited varying degrees of inhibition against six target pathogens, demonstrating broad-spectrum activity. The Bipolaris strain 2-Fr-8 and Pyrenochaeta strain 12-R-5 had strong inhibitory effects on Setosphaeria turcica and Botrytis cinerea, with inhibition rates of 50.79% and 66.67%, respectively, indicating potential biocontrol potential. The strain 11-R-10 of the genus Talaromyces exhibited strong heat resistance. Under the condition of 40 ℃, the colony diameter measured on the plate could reach 3.72 cm. Four endophytic fungi grew slowly at 4 ℃, and the colony diameter of the Fusarium strain 16-FL-1 could also reached 1.06 cm. The strain 16-FL-1 showed no difference in mycelial growth on the plate with a salt content of 3% compared to the control group, and the colony diameter could still reach 2.66 cm under the condition of 12% salt content, demonstrating strong resistance to salinization.

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备注/Memo

收稿日期：2024-11-19

基金项目：博士后落户黑龙江科研启动项目；黑龙江省现代农业产业技术协同创新推广体系-生态农业(2023-1197)；黑龙江省农业科学院农业科技创新跨越工程（CX23GG19）。
第一作者：杨帆(1982－)，女，博士，副研究员，从事有害作物病虫害生物防治研究。E-mail: yf_echo2011@163.com。
通信作者：刘春来（1975－），男，硕士，研究员，从事有害生物绿色防控研究。E-mail: liuchunlai@163.com。

更新日期/Last Update: 2025-02-10