[1]陈 平,何莉,尚 晨,等.寒地多年生麦草新种质的形态学分析与细胞学检测[J].黑龙江农业科学,2017,(03):17-22.[doi:10.11942/j.issn1002-2767.2017.03.0017]
　CHEN Ping,HE Li,SHANG Chen,et al.Cytological Detection and Morphological Analysis of the New Germplasm on Coldhardness Perennial Wheatgrass[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(03):17-22.[doi:10.11942/j.issn1002-2767.2017.03.0017]
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寒地多年生麦草新种质的形态学分析与细胞学检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年03期 页码: 17-22 栏目: 出版日期: 2017-03-10
Title:
Cytological Detection and Morphological Analysis of the New Germplasm on Coldhardness Perennial Wheatgrass
文章编号:
1002-2767（2017）03-001706
作者:
陈 平1; 何莉1; 尚 晨2; 李佶恺2; 张海玲2; 李集临1; 张延明1
1.哈尔滨师范大学 生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室，黑龙江 哈尔滨 150025；2.黑龙江省农业科学院 草业研究所，黑龙江 哈尔滨 150086
Author(s):
CHEN Ping; HE Li; SHANG Chen; LI Jikai; ZHANG Hailing; LI Jilin; ZHANG Yanming
1.College of Life Science and Technology,Harbin Normal University,Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,Harbin,Heilongjiang 150025；2.Prataculture Institute,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
寒地; 多年生麦草; 中间偃麦草; 再生性
Keywords:
cold area; perennial wheatgrass; Thinopyrum intermedium; regrowth
分类号:
S543
DOI:
10.11942/j.issn1002-2767.2017.03.0017
文献标志码:
A
摘要:
为丰富北方寒地牧草品种，选育适合北方寒冷地区种植的优势牧草，以八倍体小偃麦与中间偃麦草杂交后代选育的10份寒地多年生麦草新种质为材料，借助我国东北哈尔滨地区的气候特点，对其进行形态学分析和细胞学鉴定。结果表明：10份材料田间自然生长年限超过3a，均具有抗寒性和多年生特性，可在哈尔滨地区-30 ℃环境下安全过冬；根系发达，除2个株系8LF2114和11LF3114外，其他株系具有地下茎。植株生长繁茂，有5个株系总分孽数超过40，2个株系穗长超过24cm。株系11LF3114和11LF41181结实率平均值在80%以上，远高于亲本中间偃麦草；10份材料种子发芽率在60%~90%；株系5Q10L1181茎秆干重可达1.7 kg?m2。10个株系根尖体细胞染色体为42条，遗传稳定，植株均表现抗旱、抗寒，抗病等特性。本研究可为北方寒地的牧草选育提供理论和材料基础。
Abstract:
To enrich the northern cold pasture varieties,breeding the advantages of planting grass in the northern cold areas,the ten new germplasms of coldhardness perennial wheatgrass which selected from hybrid offsprings of Trititrigia and Thinopyrum intermedium with the climatic characteristics of Harbin located in northeast of China were analyzed and detected by the cytological and morphological method in the research.The results showed that the natural growth periods of the 10 materials were all more than 3 years,and all of them had coldresistance and perennial characteristics,even in winter of Harbin with low temperature under -30 ℃ they are safe.The roots were developed,in addition to 8LF2 114 and 11LF3 114,the other lines had underground rhizomes.The plants were in lush growth,there were 5 lines with tiller numbers more than 40 and 2 lines with spike lengths more than 24 cm.The average seed setting rate of 11LF3 114 and 11LF4 1181 was above 80%,which was much higher than parental Th.intermedium.The germination rate of the 10 seeds was between 60%~90% and the stem dry weight of 5Q10L1181 was up to 1.7 kg?m2.The somatic cell chromosomes of them were all 42,which showed genetic stability,and the resistance of drought,cold,disease and other characteristics were also presented.It is provided theoretical and material foundation for forage grass breeding in the northern cold land.

参考文献/References:

［1］ 李聪,王赞,李蕾蕾.牧草良种繁育与种子生产技术［M］.北京: 化学工业出版社,2008.
［2］ 李志勇,王宗礼.牧草种质资源描述规范和数据标准［M］.北京: 中国农业出版社,2005.
［3］ Lewandowski I,Scurlock J M O,Lindvall E,et al.The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe ［J］.Biomass and Bioenergy,2003,25(4): 335-361.
［4］ 李洪杰,朱至清.多年生农作物与环境保护［J］.华中农业大学学报,2004,34(10): 11-15.
［5］ Godfray H C J,Beddington J R,Crute I R,et al.Food Security: The challenge of feeding 9 billion people［J］.Science,2010,327:812.
［6］ Jordan N,Boody G,Broussard W,et al.Sustainable development of the agricultural bioeconomy［J］.Science,2007,316:1570-1571.
［7］ Tilman D,Socolow1 L,Foley J,et al.Beneficial biofuels the food,energy,and environment trilemma［J］.Science,2009,325(5938):270-271.
［8］ Cox T S,Glover J D,Van Tassel D L,et al.Prospects for developing perennial grain crops［J］.BioScience,2006(56):649-659.
［9］ Glover J D,Reganold J P,Bell L W,et al.Increased food and ecosystem security via perennial grains［J］.Science,2010,328: 1638-1639.
［10］Paine L K,Peterson T L,Undersander D J,et al.Some ecological and socioeconomic considerations for biomass energy crop production［J］.Biomass and Bioenergy,1996, 10(4):231-242 .
［11］ Tester M,Langridge P.Breeding technologies to increase crop production in a changing world［J］.Science,2010: 818-822.
［12］ Xu Y,Crouch J H.Markerassisted selection in plant breeding from publications to practice［J］.Crop Science,2008,48: 391-407.
［13］ 赵海滨,张延明,史春龙,等.寒地多年生小麦的选育与细胞遗传分析［J］.作物学报,2012,38(8): 1378-1386.
［14］ 靳嵩,周璇,李政宏,等.多年生小麦杂种F5代分子细胞遗传学鉴定［J］.生物技术通报,2014 (3): 65-72.
［15］ 李志勇,王宗礼.牧草种质资源描述规范和数据标准［M］.北京: 中国农业出版社,2005.
［16］ 田超,靳嵩,李集临,等.多年生作物的研究进展［J］.中国农学通报,2013,29(18):16-20.
［17］ Zhang X F,Sallamb A,Gao L L,et al.Establishment and optimization of genomic selection to accelerate the domestication and improvement of intermediate Wheatgrass［J］.Plant genome,2015,9(1).doi:10.3835/plantgenome2015.07.0059.
［18］ Kantarski T,Larson Steve L,Zhang XF,et al.Development of the first consensus genetic map of intermediate wheatgrass (Thinopyrum intermedium) using genotypingbysequencing［J］.Theoretical and Applied Genetics,2017,130(1):137-150.
［19］ Bell L W,Byrne F,Ewing M,et al.A preliminary wholefarm economic analysis of perennial wheat in an Australian dryland farming system［J］.Agricultural Systems,2008,96: 166-174.
［20］ Bell L W,Wade L J,Ewing M A.Perennial wheat: a review of environmental and agronomic prospects for development in Australia［J］.Crop and Pasture Science,2010,61: 679-690.

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备注/Memo

基金项目：国家重点研发计划资助项目 (2016YFD010010216)；黑龙江省大学生创新创业训练计划重点资助项目（201610231024）第一作者简介：陈平（1991-），男，黑龙江省齐齐哈尔市人，在读硕士，从事分子细胞遗传学研究。E-mail：1113565172@qq.com。通讯作者：张延明（1977-），男，山东省蓬莱市人，博士，副教授，从事分子细胞遗传学研究。E-mail：blueright@163.com。

更新日期/Last Update: 2017-08-06