GUAN Haiji,LI Zhigang,HE Dexin,et al.Hormonal Response and Metabolomics Analysis of Soybean Roots Under Low Nitrogen Stress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2026,(04):16-24.[doi:10.11942/j.issn1002-2767.2026.04.0016]
低氮胁迫下大豆根系的激素响应及代谢组学分析
- Title:
- Hormonal Response and Metabolomics Analysis of Soybean Roots Under Low Nitrogen Stress
- 文章编号:
- 3
- Keywords:
- soybean; low nitrogen stress; root system architecture; endogenous hormones; metabolomics; differential metabolites
- 文献标志码:
- A
- 摘要:
- 为探明不同耐低氮性大豆材料响应低氮胁迫的生理机制及关键差异代谢物调控规律，以耐低氮型大豆东农59（Res）和氮敏感型昌吉黄豆（Sus）为试验材料，通过大田试验设置常氮与低氮处理，系统测定大豆苗期根系构型、花期内源激素含量及差异代谢物特征，解析低氮胁迫下二者的响应差异。结果表明：东农59通过扩大根系表面积、增加根尖数提升氮吸收效率，其根系生长指标、叶绿素含量及根冠比显著优于昌吉黄豆；内源激素层面，耐低氮品种东农59通过上调GA3含量并维持关键激素比值的动态平衡，协同提升根系扩展与抗氧化能力，而昌吉黄豆调控能力较弱。代谢组学分析共筛选出3 131个差异代谢物（其中品种间核心差异代谢物2 187个），东农59响应更旺盛，其中脂质类代谢物（如磷脂酰胆碱）可维持细胞膜结构稳态，黄酮类代谢物（如氧化白毛茛碱、淫羊藿素）可清除活性氧以缓解氧化损伤，氨基酸类代谢物（如L-谷氨酸、L-丝氨酸）则直接支撑根系生长；氨酰-tRNA生物合成等核心通路显著富集，且差异代谢物与根系表型显著关联。相关性分析证实，L-谷氨酸等氨基酸代谢物与根系生长正相关，琥珀酸等负相关，体现代谢网络对表型适应性的支撑作用。综上，耐低氮大豆通过形态构型优化、激素稳态维持及代谢通路定向重塑的多层面协同调控，构建了以高效氮吸收为核心的低氮适应体系，本研究为耐低氮大豆品种的培育与改良提供了重要理论依据及代谢标志物。
相似文献/References:
[1]吕静,韩莉梅,张所有.超氧化物歧化酶模拟物（SODM）对大豆保护酶系统的影响[J].黑龙江农业科学,2013,(01):23.
LYUJing,HANLi-mei,ZHANGSuo-you.EffectsofSuperoxideDismutaseMimics(SODM)onSoybeanProtectiveEnzymeSystem[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(04):23.
[2]吴俊彦,于晓光.黑河地区灾后补种大豆品种筛选试验[J].黑龙江农业科学,2014,(07):13.
WU Jun yan,YU Xiao guang.Soybean Varieties Screening Test for Reseeding in Heihe Region after Disaster[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):13.
[3]安咏梅,王家军,李进荣,等.大豆抗胞囊线虫的分子标记研究[J].黑龙江农业科学,2014,(07):15.
AN Yong mei,WANG Jia jun,LI Jin rong,et al.Molecular Marker Assisted Selection on Soybean Resistant to Soybean Cyst Nematode[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):15.
[4]刘兴龙,王克勤,朱立新,等.大豆蚜对大豆阶段性危害研究[J].黑龙江农业科学,2014,(06):57.
LIU Xinglong,WANG Keqin,ZHU Lixin,et al.Study on Effect of Soybean Aphid on Soybean Growth at Different Stages[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):57.
[5]赵 寅.富裕县大豆生产现状及发展建议[J].黑龙江农业科学,2014,(05):150.
ZHAO Yin.Production Situation and Suggestions of Soybean in Fuyu County[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):150.
[6]郑 旭,赵秀梅,王连霞,等.25%阿维〖DK1〗·福〖DK1〗·莠锈悬浮种衣剂病害防效试验[J].黑龙江农业科学,2014,(05):54.
ZHENG Xu,ZHAO Xiu-mei,WANG Lian-xia,et al.Field Efficacy Trial of 25% Abamectin·Thiram·Carboxin FSC Against Soybean Disease[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):54.
[7]袁明. 齐齐哈尔大豆综合试验站现代农业产业示范基地建设成效的探讨[J].黑龙江农业科学,2013,(01):143.
YUANMing.DiscussiononConstructionEffectofQiqiharSoybeanComprehensiveExperimentalStationofModernAgriculturalIndustrializationDemonstrationBase[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(04):143.
[8]李亮,李泽宇,王丽娜,等.不同大豆品种耐盐性表现分析[J].黑龙江农业科学,2013,(02):17.
LI Liang,LI Ze-yu,WANG Li-na,et al.Performance Analysis on Salt Tolerance for Different Soybean Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(04):17.
[9]郑新利,张丙双,寇贺.东北地区大豆生产主要问题及发展对策[J].黑龙江农业科学,2012,(02):146.
ZHENG Xin-li,ZHANG Bing-shuang,KOU He.Main Problems and Developing Countermeasuresof Soybean in Northeast China[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):146.
[10]孙羽,冯延江,王麒,等.玉米茬原垄卡种大豆对其生长及产量的影响[J].黑龙江农业科学,2014,(03):26.
SUN Yu,FENG Yan jiang,WANG Qi,et al.Effect of Original Ridge Tillage on the Growth and Yield of Soybean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):26.
备注/Memo
收稿日期：2025-12-24