HE Jiahua,MAO Honglin,ZOU Jinghui,et al.Identification of Main Effect QTL for Cold Tolerance in Rice Seedling Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(06):9-13,21.[doi:10.11942/j.issn1002-2767.2025.06.0009]
水稻苗期耐冷主效QTL的鉴定
- Title:
- Identification of Main Effect QTL for Cold Tolerance in Rice Seedling Stage
- 文章编号:
- 2
- Keywords:
- rice; seedling stage; cold resistance; QTL
- 文献标志码:
- A
- 摘要:
- 水稻苗期遭遇低温冷害可导致幼苗死亡或生长停滞，严重影响水稻的产量和质量，筛选水稻耐冷资源，并解析其耐冷机制，有利于水稻耐冷育种。以冷敏品种稻花香2号为母本，耐冷品种龙粳21为父本，衍生得到包括530个株系的F23群体，经低温胁迫，从中选择30个极端耐冷和30个极端冷敏感个体构建两个DNA池进行重测序分析，使用SNP-index方法鉴定到5号染色体和7号染色体存在4个QTL区间。其中5号染色体发现了3个QTL，区间分别为3.8 Mb（4.4~8.2 Mb）、1.2 Mb（10.1~11.3 Mb）和1.6 Mb（12.2~13.8 Mb），7号染色体发现了一个QTL，区间为 2.4 Mb （2.8~5.2 Mb），功能注释分析发现，这些区间内的基因主要参与能量代谢。
- Abstract:
- Cold damage to rice seedlings at the seedling stage can lead to seedling death or growth stagnation, which seriously affects the yield and quality of rice. Screening cold-tolerant resources and analyzing their cold-tolerance mechanisms are conducive to cold-tolerant breeding in rice. In this study, we used the cold-sensitive variety Daohuaxiang 2 as the female parent and the cold-tolerant variety Longjing 21 as the male parent to derive an F2:3 population comprising 530 lines, from which 30 extreme cold-tolerant and 30 extreme cold-sensitive individuals were selected to construct two DNA pools for resequencing analysis. Two QTL intervals on chromosome 5 and chromosome 7 were identified by SNP-index method. Three peaks were identified on chromosome 5, spanning 3.8 Mb (4.4-8.2 Mb), 1.2 Mb (10.1-11.3 Mb), and 1.6 Mb (12.2-13.8 Mb), and one peak was identified on chromosome 7, spanning 2.4 Mb (2.8-5.2 Mb), and functional annotation analyses revealed that genes within these intervals mainly involved in energy metabolism, and this study lays the foundation for subsequent exploration of cold tolerance gene mining in rice.
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备注/Memo
收稿日期：2024-11-23