GUO Yaning,ZHAO Zhenyang,LIANG Mengting,et al.Effects of Auxin IAA Alone or in Combination with Cytokinin 6-KT on Growth of Hygrophila polysperma in vitro Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(09):57-64.[doi:10.11942/j.issn1002-2767.2024.09.0057]
生长素与细胞分裂素单独或组合使用对红丝青叶组培苗生长的影响
- Title:
- Effects of Auxin IAA Alone or in Combination with Cytokinin 6-KT on Growth of Hygrophila polysperma in vitro Tissue Culture
- 文章编号:
- 9
- Keywords:
- tissue culture; Hygrophila polysperma; IAA; 6-KT
- 文献标志码:
- A
- 摘要:
- 为探究生长素和细胞分裂素对水生植物组培苗的作用规律，研究了生长素(IAA、IBA和2,4-D)与细胞分裂素(6-KT)对典型水生观赏植物红丝青叶(Hygrophila polysperma)的影响。结果表明，组培培养基中单独添加不同浓度梯度的IAA、IBA、2,4-D或6-KT，组培苗在各种激素处理下，随着浓度的升高，均表现出先促进后抑制的表型，其中 IAA组表现显著，因此进一步研究IAA与6-KT的组合使用对组培苗的影响。组合添加IAA与6-KT处理18 d后，随着处理浓度的升高，整体有促进组培苗生长的趋势，其中1.0 μg·μL-1IAA+1.0 μg·μL-16-KT的处理效果最好，但随着 6-KT浓度的升高，组培苗的叶片更为膨大。组培苗在培养瓶中继续生长67 d后，较高浓度的IAA+6-KT组合的叶片出现卷曲，底部变焦枯。其中1.0~2.0 μg·μL-1IAA+2.0 μg·μL-16-KT组合使其组培苗分孽较多，叶片较大，特别是2.0 μg·μL-1IAA+2.0 μg·μL-16-KT的处理，进一步检测叶片的可溶性物质含量，发现该组合叶片的丙二醛含量较高，脯氨酸含量较低。综上可知，植物激素在红丝青叶的组织培养中应用浓度不宜过高，1.0 μg·μL-1IAA+1.0 μg·μL-16-KT组合短期处理效果最适宜。
- Abstract:
- In order to uncover the influences of auxin and cytokinin on tissue culture seedlings of aquatic plants, in this study, the single and combined effects of auxin IAA and cytokinin 6-KT were investigated on the typical ornamental plants Hygrophila polysperma.The results showed that, firstly, IAA, IBA, 2,4-D or 6-KT had an enhanced effects for each on the growth of the plantlets in vitro, and then inhibited them along with the increaseof the concentration of IAA, IBA, 2,4-D or 6-KT. Among them, 1.0 μg·μL-1IAA showed a significant change, and the combination of IAA and 6-KT was further investigated. As a result, with the increase of the concentration of IAA combined with 6-KT, the growth of the plantlets was promoted as a whole, and the treatment of 1.0 μg·μL-1IAA + 1.0 μg·μL-16-KT had the best effect, however, with the increase of 6-KT concentration, the leaf area of the plantlets obviously expanded. After 67 days treatment of high concentration of IAA + 6-KT, the leaves of H. polysperma appeared to be coiled and the bottom of the leaves became scorched.The combination of 1.0-2.0 μg·μL-1IAA + 2.0 μg·mL-16-KT made the leaves branched and large, especially, under the combinationof 2.0 μg·μL-1IAA + 2.0 μg·mL-16-KT, the leaves of plantlets had higher malondialdehyde and lower proline content relative to other groups. In conclusion, the application concentration of plant hormonesin tissue culture of aquatic plants is not feasible to be too high, and the combinationof 1.0 μg·μL-1IAA+1.0 μg·μL-16-KT is appropriate for short-term treatment.
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备注/Memo
收稿日期：2024-04-17