[1]金羽,曲娟娟,闫立龙,等.小白菜联合铜绿假单胞菌对田间铅污染土壤的修复[J].黑龙江农业科学,2013,(08):32-36.
　JIN Yu,QU Juan-juan,YAN Li-long,et al.Field Test of Bio-remediation of Lead Polluted Soil by Pseudomonas aeruginosa Strain M2 Combined with Pakchoi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):32-36.
点击复制

小白菜联合铜绿假单胞菌对田间铅污染土壤的修复

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年08 页码: 32-36 栏目: 出版日期: 2013-08-31
Title:
Field Test of Bio-remediation of Lead Polluted Soil by Pseudomonas aeruginosa Strain M2 Combined with Pakchoi
文章编号:
1002-2767（2013）08-0032-05
作者:
金羽1; 2; 曲娟娟1; 闫立龙1; 孙兴滨3
1.东北农业大学 资源与环境学院，黑龙江 哈尔滨 150030；
2.哈尔滨工业大学 市政与环境工程学院，黑龙江 哈尔滨 150090；
3.东北林业大学 林学院，黑龙江 哈尔滨 150040
Author(s):
JIN Yu1; 2; QU Juan-juan1; YAN Li-long1; SUN Xing-bin3
1.College of Resources and Environmental science,Northeast Agricultural University,Harbin 150030;
2.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin，Heilongjiang 150090;
3.College of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040
关键词:
小白菜; 铜绿假单胞菌; Pb污染; 生物修复
Keywords:
pakchoi; Pseudomonas aeruginosa; Pb pollution; bio-remediation
分类号:
X53
文献标志码:
A
摘要:
针对土壤中铅(Pb)污染的生物修复，在种植小白菜的田间Pb处理土壤中，接种1株耐Pb铜绿假单胞菌(Pseudomonas aeruginosa)M2菌株，研究其对小白菜吸收Pb的影响。结果表明:接种M2可以降低土壤Pb污染对小白菜生长的抑制作用，促进小白菜对土壤Pb的吸收，使接种组小白菜生物量和株高分别增加7%和12%，根际土壤Pb残留量降低7%左右；接种处理的土壤脲酶和转化酶活性在小白菜整个生长期内均较未接种组高。结合前期盆栽试验结果得出，接种耐铅铜绿假单胞菌M2，可以促进小白菜对Pb污染土壤的修复。
Abstract:
For the bio-remediation of Pb pollution in soil,a strain of Pb tolerant bacterium,Pseudomonas aeruginosa M2 was inoculated to field cultivated pakchoi and its effects on the lead absorption by pakchoi in Pb contaminated soil was investigated.The results showed that：M2 vaccination could reduce the inhibition of soil Pb pollution on the growth of pakchoi and promote absorption of Pb in soil,the plant biomass and height were increased by 7% and 12%,respectively.At the same time,the soil Pb residual quantity in rhizosphere reduced about 7% compared with non-inoculated groups.Throughout the growth period of pakchoi,for the inoculated groups,the soil urease and invertase activities were increased.In conclusion,inoculating with Pb tolerant bacterium Pseudomonas aeruginosa  M2 to Pb polluted soil could promote the bio-remediation of Pb by pakchoi in soil.

参考文献/References:

[1]黄鸿翔.我国土壤资源现状、问题及对策[J].土壤肥料，2005（1）:3-6.
[2]赵庆龄，张乃弟，路文如.土壤重金属污染研究回顾与展望Ⅱ：基于三大学科的研究热点与前沿分析[J].环境科学与技术，2010，33(7):102-106.
[3]崔德杰,张玉龙.土壤重金属污染现状与修复技术研究进展[J].土壤通报，2004，35(3):366-370.
[4]腾应，骆永明，李振高.污染土壤的微生物修复原理与技术进展[J].土壤，2007，39(4):497-502.
[5]Sun R L,Zhou Q X,Jin C X.Cadmium accumulation in relation to organic acid in leaves of  Solanum nigrum  L.as a newly found cadmium hyperaccumulator[J].Plant and Soil,2006,185:125-134.
[6]廖晓勇，陈同斌，阎秀兰，等.提高植物修复效率的技术途径与强化措施[J].环境科学学报，2007，27(6):881-893.
[7]江春玉，盛下放，何琳燕，等.一株铅镉抗性菌株WS34的生物学特性及其对植物修复铅镉污染土壤的强化作用[J].环境科学学报，2008，28(10):1961-1968.
[8]Chen Y X,Wang Y P,Lin Q,et al.Effect of copper-tolerant rhizosphere bacteria on mobility of copper in soil and copper accumulation by Elsholtzia splendens[J].Environment International,2005,31:861-866.
[9]陈宝，徐晓萌，曲娟娟，等.铜绿假单胞菌M2联合小白菜对Pb污染土壤的生物修复[J].浙江大学学报：农业与生命科学版，2012，38(6)：732-740.
[10]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:45-60.
[11]Whiting S N,De S,Terry N.Rhizosphere bacteria mobilize Zn for hyperaccumulation by Thlaspi caerulescens[J].Environmental Science and Technology,2001,35(15):3144-3150.
[12]陈素华,孙铁珩,周启星，等.微生物与重金属的相互作用及其应用研究[J].应用生态学报,2002,13(2):239-242.
[13]De Souza M,Huang C,Chee N,et al.Rhizosphere bacteria enhance the accumulation of selenium and mercury in wetland plants[J].Planta,1999,209(2):259-263.
[14]刘敬武，单爱琴，揣小明.重金属离子Cd2+、Pb2+污染对土壤酶活的影响[J].污染防治技术,2008,21(1):19-22.
[15]段学军，闵航.Cd胁迫下稻田土壤生物活性与酶活性综合研究[J].农业环境科学学报，2004，23(3):422-427.
[16]李廷强，朱恩，杨肖娥，等.超积累植物东南景天根际土壤酶活性研究[J].水土保持学报，2007，21(3):112-117.
[17]王建坤，张小平，周薇.铅锌矿区土壤微生物区系及酶活性调查[J].环境监测管理与技术，2009，21(4):23-27.

相似文献/References:

[1]赵索,陈丽,周传余,等.小白菜品系的耐热性鉴定[J].黑龙江农业科学,2014,(07):86.
　ZHAO Suo,CHEN Li,ZHOU- Chuan yu,et al.Identification of Heat Resistance of Brassica rapa L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):86.
[2]张玉磊.GT-S叶面肥对小白菜产量及品质的影响[J].黑龙江农业科学,2016,(03):44.[doi:10.11942/j.issn1002-2767.2016.03.0044]
　ZHANG Yu-lei.Effect of GT-S Foliar Fertilizers into Surface of Leaves on Yield and Quality of Chinese Cabbage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(08):44.[doi:10.11942/j.issn1002-2767.2016.03.0044]
[3]刘挺君,李君昭,洪若晖,等.苯酚对小白菜幼苗株高及生理特性的影响[J].黑龙江农业科学,2017,(11):33.[doi:10.11942/j.issn1002-2767.2017.11.0033]
　LIU Ting-jun,LI Jun-zhao,HONG Ruo-hui,et al.Effect of Phenol on Seeding Height and Physiological Characteristics of Chinese Cabbage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(08):33.[doi:10.11942/j.issn1002-2767.2017.11.0033]
[4]田 莹,孙伟娜,林志伟,等.白僵菌几丁质培养液对小白菜抗逆酶及土壤微生物的影响[J].黑龙江农业科学,2018,(01):63.[doi:10.11942/j.issn1002-2767.2018.01.0063]
　TIAN Ying,SUN Wei-na,LIN Zhi-wei,et al.Effects of the Chitin Medium with Beauveria bassiana on Anti Reverse Enzyme of Chinese Cabbage and Soil Microorganism[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(08):63.[doi:10.11942/j.issn1002-2767.2018.01.0063]
[5]文安宇,陈阳,王雪华,等.生物菌剂对小白菜生长及品质的影响[J].黑龙江农业科学,2019,(06):69.[doi:10.11942/j.issn1002-2767.2019.06.0069]
　[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):69.[doi:10.11942/j.issn1002-2767.2019.06.0069]
[6]孙佳玉,吴瑕,王鹏,等.不同鸡粪用量对小白菜干物质积累和养分吸收的影响[J].黑龙江农业科学,2019,(12):65.[doi:10.11942/j.issn1002-2767.2019.12.0065]
　[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):65.[doi:10.11942/j.issn1002-2767.2019.12.0065]
[7]杨晓磊,高文,彭衍彪.蚯蚓生物有机肥对小白菜生长和品质的影响[J].黑龙江农业科学,2021,(08):34.[doi:10.11942/j.issn1002-2767.2021.08.0034]
　YANG Xiao-Lie,GAO Wen,PENG Yan-biao.Effects of Earthworm Biological Organic Fertilizer on Growth and Quality of Pakchoi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(08):34.[doi:10.11942/j.issn1002-2767.2021.08.0034]

备注/Memo

收稿日期：2013-07-01
基金项目：环保公益性行业科研专项资助项目(2010467038)
第一作者简介：金羽（1979-），女，黑龙江省伊春市人，博士，讲师，从事环境生物技术研究。E-mail:jinyu1022@126.com。
通讯作者：曲娟娟（1974-），女，山东省龙口市人，博士，教授，博士生导师，从事生物修复，废弃物处理与资源化研究。E-mail:juanjuanqu@126.com。

更新日期/Last Update: 2014-10-30