ZHANG Suxin.Differences in Resistance to Metazosulfuron Among Different Rice Varieties and Underlying Mechanisms[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(07):28-34.[doi:10.11942/j.issn1002-2767.2025.07.0028]
不同水稻品种对嗪吡嘧磺隆的耐药性差异及其机制
- Title:
- Differences in Resistance to Metazosulfuron Among Different Rice Varieties and Underlying Mechanisms
- 文章编号:
- 5
- 关键词:
- 水稻; 嗪吡嘧磺隆; 耐药性; 谷胱甘肽-S-转移酶; 乙酰乳酸合成酶
- Keywords:
- rice; metazosulfuron; resistance; GSTS; ALS
- 文献标志码:
- A
- 摘要:
- 为探索黑龙江省不同水稻品种的耐药性，以黑龙江省主栽的12个水稻品种为研究对象，测定嗪吡嘧磺隆对不同水稻品种的根长抑制率以及对GSTs、ALS和抗氧化酶（SOD、POD、CAT）活性的影响。结果表明，不同水稻品种对嗪吡嘧磺隆的耐药性有较大差异，其中五优稻4号最为敏感，抑制中剂量（ED50值）仅为0.060 4 g·hm-2，龙粳31耐药性最强，ED50值为0.335 2 g·hm-2，耐药性指数可达5.55。12个水稻品种对嗪吡嘧磺隆的耐药程度依次为龙粳31＞松粳28＞绥粳18＞龙粳21＞盛誉1号＞松粳60＞绥粳27＞龙稻1604＞龙稻18＞龙粳47＞龙粳1624＞五优稻4号。嗪吡嘧磺隆可诱导水稻体内GSTS活性增加，施药后龙粳31和五优稻4号体内GSTS活性均呈先升高后降低趋势，药后10 d，龙粳31体内GSTS活性可降低至对照水平，而五优稻4号仍显著高于对照，恢复速度低于龙粳31。嗪吡嘧磺隆能够降低龙粳31和五优稻4号体内ALS酶活性，龙粳31可于药后5 d恢复至对照水平，而五优稻4号药后5 d体内ALS水平仍显著低于对照，恢复速度低于龙粳31。嗪吡嘧磺隆能够激活龙粳31和五优稻4号体内抗氧化系统，龙粳31体内抗氧化酶SOD、POD、CAT活性均能够快速升高以抵抗氧化应激，并在药后10 d恢复正常，而五优稻4号抗氧化系统响应缓慢，药后10 d体内抗氧化酶活性仍未恢复至正常水平。不同水稻品种GSTS、ALS及抗氧化酶活性存在差异，施药后耐药水稻体内酶活恢复速度显著高于敏感水稻，推测其中GSTS及抗氧化酶活性响应差异是导致不同水稻品种对嗪吡嘧磺隆耐药性差异的主要原因。
- Abstract:
- In order to explore the resistance of different rice varieties in Heilongjiang Province, in this study, 12 main rice varieties cultivated in Heilongjiang Province were selected as the research subjects. The effects of metazosulfuron on rice root length inhibition and enzyme activities of GSTs, ALS and antioxidant enzymes(SOD, POD, CAT) were determined. The results showed that different rice varieties showed different resistance to metazosulfuron, with Wuyoudao 4 being the most sensitive, ED50 value of only 0.060 4 g·ha-1, Longjing 31 and the most resistant, ED50 value of 0.335 2 g·ha-1, and resistance index up to 5.55. The resistance of the 12 rice varieties to metazosulfuron was Longjing 31> Songjing 28> Suijing 18> Longjing 21> Shengyu 1> Songjing 60> Suijing 27> Longdao 1604> Longdao 18> Longjing 47> Longjing 1624> Wuyoudao 4. Metazosulfuron reduced GSTS activity in rice, after application, the GSTS activity of Longjiang 31 had Wuyoudao 4 rice plants increased first and then decreased. At 10 days after the drug, the GSTS activity of Longjing 31 was reduced to the control level, while Wuyoudao 4 was still significantly higher than that of the control. Metazosulfuron was able to reduce ALS enzyme activity in rice leaves, and Longjing 31 could recover to the control level at 5 days after the drug, while the level of ALS in Wuyoudao 4 was still significantly lower than that of the control group on the 5 days after the treatment. Metazosulfuron can activate the antioxidant systems in both rice varieties. In the rice Longjing 31, the activities of antioxidant enzymes (SOD, POD, CAT) rapidly increase to counteract oxidative stress, returning to normal levels by 10 days after herbicide application. In contrast, the antioxidant system in the rice Wuyoudao 4 responds sluggishly, with its antioxidant enzyme activities remaining unrecovered even 10 days post-treatment. GSTS, ALS and antioxidant enzymes activities were different among different rice varieties, the recovery rate of enzyme activity in resistant rice was significantly higher than that of sensitive rice，and the differences in the responese of GSTS and antioxidant enzyme activity was the main reason for the difference in resistance to different rice varieties.
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备注/Memo
收稿日期：2025-04-03
基金项目：黑龙江省农业科学院植物保护研究所青年基金项目（zbsqn2024-2）。
作者简介：张苏新（1997－），女，硕士，研究实习员，从事有害生物综合防治研究。E-mail: 574982719@qq.com。