HAN Xiao-yun-,ZHENG Gui-hua-,ZHENG Bao-jiang-,et al.Isolation and Identification of Pseudomonas sp.H8 with Flocculant-producing Capacity and Analysis of the Flocculant[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(09):115-120.[doi:10.11942/j.issn1002-2767.2019.09.0115]
Pseudomonas sp.H8的分离和鉴定及其产絮凝剂成分分析
- Title:
- Isolation and Identification of Pseudomonas sp.H8 with Flocculant-producing Capacity and Analysis of the Flocculant
- Keywords:
- low temperature flocculants-producing bacteria screening; strain identification; strain H8-derived flocculants; component analysis
- 文献标志码:
- A
- 摘要:
- 为挖掘产生物絮凝剂的菌株，利用生物絮凝剂对污水、淤泥进行环保处理。本研究经过菌种分离纯化和对高岭土悬浊液沉降效果分析，从低温污水处理生物膜中获得1株高效絮凝菌，命名为H8，该菌株在4 ℃条件下生长良好，其LB发酵液对高岭土的絮凝效率达到85.79%，针对H8菌株的一系列菌种鉴定，包括菌落形态、菌体形态、生理生化试验、Biolog菌种鉴定系统分析和细菌16S rDNA序列分析比对，与菌种Pseudomonas mandelii亲缘关系最近，确定H8菌株为假单胞菌属。采用乙醇沉淀法对H8菌株发酵液中的絮凝剂进行提取，再经过Sevage法除去絮凝剂中蛋白质，获得絮凝剂产率0.807 g·L-1-，与发酵液相比其絮凝率也提高到98%以上，定性定量分析结果确定H8絮凝剂的化学成分主要为多糖，占51%，还含有2.64%蛋白质、1.3%-葡萄糖醛酸和少量氨基糖。
- Abstract:
- The use of bioflocculants for the treatment of sewage and sludge has such advantages as environmentally friendly and no secondary pollution.Therefore,the exploitation of flocculants-producing strains,especially those that produce flocculants at low temperature,has important significance theoretically and application prospects.In this study,the strain named H8 with efficient flocculation activities was isolated and purified from the low-temperature sewage treatment biofilm,the flocculation effect towards kaolin suspension was also evaluated.The strain grew well at 4 ℃,and its LB fermentation broth showed 85.79% flocculation efficiency towards kaolin.The H8 strain was characterized with colony and cell morphology,physiological and biochemical tests,Biolog strain identification system analysis and bacterial 16S rDNA sequence analysis,which showed close relation to Pseudomonas mandelii and identified as Pseudomonas.The flocculants in the H8 strain-derived fermentation broth was extracted by ethanol precipitation with flocculants yield of 0.807 g·L-1- after the protein removal by sevage method.The flocculation rate was also increased to 98%.Qualitative and quantitative analysis confirmed that the chemical constituents of H8 flocculants were mainly composed of polysaccharides which accounted for 51% (w/w),protein (2.64%,w/w),glucuronic acid (1.3%,w/w) and a small amount of amino sugar.
参考文献/References:
［1］-张一卉,朱迟,梁慧芳,等.微生物絮凝剂概况及发展前景[J].-生命科学研究,2005(S1):39-42.
备注/Memo
收稿日期：2019-04-17