[1]王郑昊,王传光,赵琦,等.多花兰与蕙兰杂交及种胚萌发研究[J].黑龙江农业科学,2013,(02):73-76.
　WANG Zheng-hao,WANG Chuan-guang,ZHAO Qi,et al.Study on the Crossing between Cymbidium floribundumwith Cymbidium faberiduse and the Embryo Germination[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(02):73-76.
点击复制

多花兰与蕙兰杂交及种胚萌发研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年02 页码: 73-76 栏目: 出版日期: 2013-02-28
Title:
Study on the Crossing between Cymbidium floribundumwith Cymbidium faberiduse and the Embryo Germination
文章编号:
1002-2767（2013）02-0073-04
作者:
王郑昊1; 王传光2; 赵琦1; 张林3; 李承秀3; 王长宪3
1.泰安市徂徕山林场，山东 泰安 271000；
2.泰安市气象局，山东 泰安 271000；
3.泰安市泰山林业科学院，山东 泰安 271000
Author(s):
WANG Zheng-hao1; WANG Chuan-guang2; ZHAO Qi1; ZHANG Lin3; LI Cheng-xiu3; WANG Chang-xian3
1.Taian Culaishan Forest Farm， Taian，Shandong 271000；
2.Taian Meteorological Bureau，Taian，Shandong 271000；
3.Taishan Forestry Science Institute,Taian,Shandong 271000
关键词:
多花兰; 蕙兰; 杂交; 萌发
Keywords:
Cymbidium floribundum; Cymbidium faberiduse; crossbreed; germination
分类号:
S682.31
文献标志码:
A
摘要:
为选育出有香味、观赏性好、抗性强、栽培容易的品种,以多花兰为父本、蕙兰大一品为母本进行杂交,研究人工授粉、果实生长情况,开展兰花种间杂交育种的基础研究工作,了解杂交过程中可能出现的问题;对得到的杂交种子进行非共生萌发,建立完整的非共生萌发技术体系;在杂交根状茎的培养阶段,设计不同基本培养基、激素浓度和添加物,筛选最佳根状茎增殖、分化和生根壮苗的培养基。结果表明:多花兰与蕙兰大一品杂交授粉容易,结实率高,为100%。杂交适宜采收期90～150d;接种种胚最佳萌发时间为90～150d。最佳萌发培养基为ZB11。其根状茎的最佳增殖、分化和生根培养基为:1/2MS+6-BA 0.3mg.L-1+NAA1.0mg.L-1+椰汁20mL.L-1+AC 0.5g.L-1。在试验增殖的过程中出现了较为整齐的分化和生根,分化率达到95%以上。炼苗基质以草炭∶珍珠岩∶火山石=3∶3∶4为最佳。
Abstract:
In order to breed out the varieties with good looking,strong resistance,fragrance,easy to cultivation,Cymbidium floribundum was used as male parent and Cymbidium faberiduse ‘ Dayipin’ was used as female to conduct the crossbreed interspecies.The artificial pollination and the growth of Cymbidium fruit were researched to investigate the process of the crossing.The basis research work of crossbreed of Cymbidium was carried out.The system of asymbiotic germination propagation was established through the studies of asymbiotic germination of the crossbreed seeds.The appropriate culture mediums of the rhizome proliferation，differentiation and rooting were founded through the design of different basic medium,hormone concentration and additives.The results showed that it was easy to obtain hybridization pollination of Cymbidium floribundum and Cymbidium faberiduse ‘ Dayipin’,seed setting rate was 100%.Advisable harvest time was 90 ~ 150 d for hybrid.The best embryo germination time was 90 ~ 150 d.The best medium for germination was ZB11.The best proliferation,differentiation and rooting mediums of rhizomes were 1/2MS+6-BA 0.3 mg?L-1+NAA 1.0 mg?L-1+Coconut juice 20 mL?L-1+AC 0.5 g?L-1In the test,the breeding process appeared relatively regular differentiation and rooting,differentiation rate was above 95%.The best base material of hardening-seedling was grass carbon∶perlite∶pelelith=3∶3∶4.

参考文献/References:

[1]刘仲健，陈心启，茹正忠.中国兰属植物[M].北京：北京科学出版社，2006：59-62.

[2]卢思聪，石雷.大花蕙兰[M].北京：中国农业出版社，2005：2-80.
[3]吴汉珠，王续衍，林泰碧.‘中国兰’茎顶组织培养研究[J].园艺学报，1987，14(3):203-207.
[4]Shimasaki K，Uemoto S.Micropropagation of a terrestrial Cymbidium species using rhizomes developed from seeds and pseudobulbs [J].Plant Cell Tiss Org Cult，1990，22: 237-244.

备注/Memo

收稿日期：2012-09-06

第一作者简介：王郑昊（1985-），男，山东省泰安市人，硕士，助理工程师，从事森林抚育经营工作。E-mail:andy-w-v-l@163.com。
通讯作者：王长宪（1959-），男，山东省平阴县人，硕士，研究员，硕士研究生导师，从事观赏植物育种工作。

更新日期/Last Update: 2013-02-28